( 436 ) 
but that it was present in the chyle; hence the transformation of 
fat in a mixture of blood and chyle. 
To my regret [I was obliged to cut short my investigation on 
this subject. Although [ am fully aware that these results are in- 
complete in many ways, it seemed expedient to me to publish them 
at present, as for some time I shall not have the opportunity to 
pursue this subject, and I wished to stir up other inquirers to the 
use of ascites-liquid for the study of the lipolytic ferment. The 
mucoid ascites-liquid can be had in such abundance (repeatedly more 
than 8 Liter of liquid were removed from the abdominal cavity 
of the patient), that it will afford a better and more extensive oppor- 
tunity for the study of the nature and the effects of the lipolytic 
ferment, than most other animal ferments. 
The above mentioned researches have give the following results : 
1. It is possible to make from lipanine (acid olive oil) a perfect 
dust-shaped emulsion. This has not only been successfully performed 
with the aid of the examined mucoid ascites-liquid, but also with 
ordinary horse-blood-serum. 
2. This fact seems to indicate, that during life, the transition 
into dust-shape of the small fat globules, which still exist in the 
adenoid tissue of the villi, is caused by the continuous motion of 
the lymph of the villi. 
3. The opalescent, non-fatcontaining, mucoid ascites-liquid exa- 
mined by us, contains a lipolytic ferment, which possesses the power 
to transform dust-shaped fat. For this transformation the presence 
of bloodcorpuscles and also access of air is necessary. 
4. The contention of COHNSTEIN and Mrcnaöris, that the 
lipolytic ferment discovered by them, originates from the blood, has 
not been proved. Their and my experiments rather show, that the 
ferment is a constituent of the chyle. 
