( 674 ) 
acid ferments takes rise in the medium inoculated with rough 
material; this neither reoccurs after transplantation. 
After the first or second transplantation the flora chiefly consists 
of a group of aërobie bacteria of which four species have been 
isolated; B. lipolyticum a, B, y, and d. These bacteria have the 
shape of short double rodlets, 0.15 u—t u wide and 0.25—2.5 w 
long, slightly motile and wrapped in a slimy envelope. The colonies 
show resemblance to those of B. aërogenes; often the middle part 
is somewhat elevated. On broth gelatin after 5 days’ culture at 
20° C. they grow out to white or greyish white, sometimes slimy 
colonies, which after 5 days get a diameter of 1.5—2 mm. On broth 
agar they are more transparent and flatter. 
The growth optimum is + 35° C. They cannot resist heating 
for 10 minutes at 60° They thrive better on broth gelatin or broth 
agar than on media with salts of organic acids (malic acid and 
lactic acid) as carbon source and ammonium chloratum as source of 
nitrogen. On = slices of potato these microbes grow out to white 
or greyish white moist colonies. Broth becomes very turbid after 
inoculation, at the bottom of the test tube a sediment forms, no film 
at the surface. 
They thrive very well in milk which becomes viscous, alkali 
being formed. B. Lipolyticum y and d form chymosine, the two 
others not or very little. Trypsine is not produced, neither diastase 
nor ureasa. Indican or aesculine are not split. On whey gelatine 
the growth is good and alkali is formed whereby an iridescent film 
appears. Indol is not produced, nitrate not reduced to nitrite, glucose 
is not fermented. The optimum of the lipase action lies near +65°. 
In culture liquids containing ammonium chloratum as source of 
nitrogen a good growth is obtained with the following carbon sources: 
alcohol, glycerin, glucose, saccharose calciummalate, -lactate, -steari- 
nate, aethylacetate, aethylbutyrate, tributyrine, trioleïne. 
In a culture medium of the composition: 100 tapwater, 1 fat, 
0,05 NH,CL, 0,05 K,HPO,, 1 CaCO,, after ten days’ cultivation at 
25° B. lipolyticum « and B had split respectively 130 and 105 
mGs. of fat, and assimilated 20 and 21 mGs. In brothwater, 2 °/, 
peptone, 1 °/, CaCQ,, and 1 °/, fat, in ten days respectively 630 and 
480 mGs. of fat were split, and resp. 40 and 80 mGs. were 
assimilated. 
Aërobie cultivation at 45°—55°. At these temperatures fat-splitting 
is seldom observed even with addition of large quantities of inocu- 
lation material (5 Gr.) to the medium. The cultures in which fat- 
spitting occurs contain a species closely allied to L. mesentericus 
