( 698 ) 
Effect on intact animals. 
Frogs were subcutaneously injected with derrid (0,5—6 m.g.); “normal 
animals” were given corresponding quantities of alcohol. Temporariae 
and esculentae did not show any change. After an injection of 3 m.g. 
nothing particular could be observed; all reflexes were present, 
while on stimulation of the spinal cord the legs were put out when 
a certain position of the secondary coil was reached; heart-and 
respiration-movements were Clearly visible. After 3 hours an animal 
could stand supination; the head-reflex when the whole frog was 
horizontally turned round, had disappeared; the sensitiveness of the 
legs was still present; the position of the secondary coil, on stimulation 
of the spinal cord, was still the same; the motion of the heart was 
clearly visible, but respiration-movements could no longer be observed. 
After 6 hours the sensitiveness had disappeared; after 9 hours 
heart-movements could be observed no more. The autopsy showed 
that the heart was in half-systoie, which was not always the case. 
If a muscle-nerve preparation was made, it appeared that nerve and 
muscles were still sensitive; only in the place of injection the muscle 
was not sensitive and looked as if boiled. This could not be put down 
to alcoholic action, which appeared from experiments of comparison. 
In other experiments defective jumping and other coordination- 
disturbances could be observed. 
From these and other experiments it appears that first brain- 
narcosis and paralysis of the respiratory-centre manifest themselves ; 
then at last heart-paralysis. In all these cases the clectrical sensitiveness 
of the spinal cord, the motor nerves and muscles remained unchanged 
till the last (except in the place of injection). 
White mice, subcutaneously injected with 0,01—38 mg. did not 
show reaction until dosed with 0,1 mg., from which they recovered. 
Action of alcohol was excluded. 
A mouse, injected with 0,5 m.g. reacted after 1'/, hours on 
touching the cornea and pinching its tail; respiration visible; heart- 
beat could be felt; after 3'/, hours the rate of the respiration had 
considerably lowered ; rate of the pulse unchanged ; after 5 hours it could 
stand being put in all positions, reacted upon pinching its tail and its 
legs; after 8 hours no signs of life could be observed; during the 
autopsy muscular movements showed themselves and the heart was 
sull beating; the latter showed the changes previously mentioned. 
In other cases we saw a kind of catalepsy: when put with two legs 
on the edge of a table it remained sitting, while its other legs were 
hanging over the edge and its tail was held quite straight. 
