( 989 ) 
Our views were moreover confirmed by an experiment, tending 
to investigate to what extent the phagocytosis could be revived in 
an 18 hour old leucocyte suspension by the iodoform-solution which 
had been exposed to the light. In the same experiment the effect of 
the fresh iodoform-solution was ascertained and also that of chloride 
of caleium. 
At the same time the effect was investigated of a NaCl-solution, 
containing a slighter amount of the old iodoform, and which had 
been obtained by diluting the old saturated iodoform solution with 
double the volume of a NaCl-solution. This experiment we resolved 
upon with a view to the consideration that if the old iodoform 
solution contained a noxious substance it would make itself less felt, 
if this solution were diluted. Under these circumstances, the favour- 
able effect of that iodeform in the solution, which had not been 
decomposed, would more plainly manifest itself. 
Tt ACBL Ee MIL 
Effect of fresh and old iodoform-solutions and of CaCl, on 
the almost paralyzed leucocytes. 
a Percentage of leucocytes 
Eid: having taken up carbon. 
19 
NaCl 0,9 Vy | AGL ed 100 — 4A, 
| / 
Fresh saturated solu- | ie 
tion of lodoform in | 153 S< 100 = 45,6 
NaCl 0,90/, | 976 a 
(0.001' /o. CHL) 
Old saturated solution rd 0 
of Iodoform in : | — X10= 0 , 
NaCl 0,9/, \ | 624 
| vol. of the old saturated 
solution of lodoform in | ~( 
NaCl 0,99, and 2 vol. | 100 = 12,7, 
NaCl 0,9°/, | HUA Mat 
(0.0003°/,, CHI,) 
NaCl solution 0.9"), Se. . 4 
+ 0,050/, CaCl, DOD x badend Lid 
We see from this experiment: 
1. that the phagocytosis in the old leucocyte suspension has fallen 
to about 4°/,; 
2. that the fresh iodoform solution raises the phagocytarian power 
again to 15.6°/,, but that 
65 
Proceedings Royal Acad. Amstefdam. Vol. XIII. 
