( 1003 ) 
salts, and also to some other salts, the faculty of rendering inactive 
pancreatic-juice, or pancreatic extract, active with respect to albumen, 
in other words, capable of activating trypsinogen. 
During the last few months | too have made a number of 
experiments with a view to obtaining, if possible by an independ- 
ent investigation, a confirmation of the results arrived at by these 
authors, whose conclusions are for the rest pretty well identical. | 
became aware that an investigation as to the activating effect of 
chloride of calcium on trypsinogen (I have not as yet extended my 
investigation to other salts) though apparently simple, was attended 
with considerable difficulties. Hence my experiments on the subject 
have not vet led to any definite result so far as the activating effect 
of chlorid of calcium on trypsinogen is concerned. But in the course 
of this investigation some other facts have come to light whieh 
seem to me to be worth publishing, the more so as the difficulties 
encountered, will be to some extent set forth and explained by them. 
In the course of the above-mentioned investigation it was namely 
discovered that chloride of calcium has the property of materially 
stimulating the action of trypsin, when already active in itself 
and free from trypsinogen. The same fact was observed about intes- 
tinal mucous membrane extracts, but in a much slighter degree. 
There is no need to point out that activating trypsinogen (i. e. 
transforming inactive trypsinogei into active trypsin) and stimulating 
the action of trypsin (i.e. stimulating into greater activity the ferment 
when free from trypsinogen and already active as it is) are two 
entirely different notions. 
It has already been said that a further investigation relating 
to this stimulating action was begun on account of observations 
made in some experiments, which were carried out to study the 
activating effect of chloride of calcium on trypsinogen. We subjoin 
some of the experiments made. (Tables I and Il). Beforehand it 
must be mentioned that the pancreatic-juice experimented with, was 
obtained by pressing out a pig’s pancreas, likewise that the extracts 
from the intestinal mucous membrane were prepared by extracting 
the scraped-off intestinal mucous membrane, and filtrating it after 
some time. Further that in all the experiments discussed in this 
paper, we used for albumen: coagulated white of hens’ eggs, accord- 
ing to Merr’s well tried method, and finally that wherever in this 
composition chloride of calcium is spoken of, the salt without water 
is meant. 
From the experiment detailed in Table 1 we may draw the fol- 
lowing conclusions: 
