( 1004 ) 
TA BAE. 1 
Digestion of the egg- 
white-columns in 
m.m. after 
24 hours 2 > 24 hours 
| Pancreatic juice: 3 drops + 2), NaFl-solution : 5 c.c. 8 ES 
2» a ay » -+ extract in 2 /, NaFl sol.: 
Dace 12 22 
3 Le :3 _„ -+ boiled extract in 2'/9 NaFl 
sol. 5 C.C, 9 19 
4 FA wes. » —+ distilled water: 5 c.c. Ba 12 
5 . ee) „ +0.49/, CaCl, sol.: 2} c.c. | 
| + water: 24 c.c. 2 4 25 
6 | : 3 +10 CaCl sol.: 24 éc. | | 
| -+ water: 23 c.c. | 12 26 
| 
7 Me ee se Re oe, 
+ 20/) NaFI sol.: 2} c.c. | 6 | & 
| | 
In the first place it appears from 1) that the pancreatic-juice con- 
tained trypsin, for we observe that in a medium of a 2°/, sol.Nakl 
a not inconsiderable digestion had taken place. If the pancreatic-juice 
had been entirely free from trypsin, in other words if it had con- 
tained nothing but trypsinogen, then no digestion of albumen would 
have taken place in a medium of a 2°/, NafFl-solution. From a 
comparison between 1) and 2) it appears that in 2) the albumen- 
digestion is greater than in 1). It foliowed from this that probably 
not all trypsinogen had passed into trypsin, because it had to be 
assumed that in 2) trypsinogen had still been activated by the ex- 
tract from the intestinal mucous membrane. 
Since this pancreatic-juice contained already free trypsin, we 
evidently could draw no conclusions from it as to trypsinogen being 
activated by chlorid of calcium; from this point of view, therefore, 
this experiment had to be looked upon as having failed. 
On comparing +) with 1) we see that after after 48 hours the 
digestion in 4) is considerably less than in 1). This result must be 
attributed to the fact that in 1) the bacteria-development was impe- 
ded by the Nall-solution, whereas in 4) the bacteria were able to 
develop themselves freely. 
Bacteria (at least some bacteria, I shall, however, not enter into 
this subject, as it does not bear on the matter under consideration) 
