( 1006 ) 
emall, whilst as regards trypsin, the antitryptic effect of the bacteria 
would have manifested itself in 5) and 6) as strongly as in 4), if 
not another factor had been present, which promoted in 5) and 6) 
the trypsin-action more than it was counteracted by the bacteria. 
These considerations suggested the idea to me that chlorid of cal- 
cium might perhaps have the property of sumulating the activity of 
trypsin. 
This would, moreover, explain the results of these, and such like 
experiments, for I repeatedly found similar results. 
As a type of the experiments, in which the pancreatic juice used, 
was free from trypsin, containing nothing but trypsinogen, we may 
quote the following (Table II). 
TABLE AL 
mmm 
‚_ Digestion of two 
albumen-columns in 
Fluids added Ee 
‘Amount of Pancreatic- | 
juice used 24 hrs. | 2X 24 hrs. 
1) 2 drops + 2, NaFl-solution: 10 cc, 0 0 
+ intestinal mucous membrane 
2) 2 a extract in 2’, NaFl-solution: 
10e 54 9 
3) 2 2 + boiled intestinal mucous mem- 
brane extract in 2°, NaFl- 
solution: 10 c.c. 0 0 
4) 2 d 
+. 19 chlorid of calcium-solu- | 
tion: 5 c.c. + 2°), NaFl-solu- 
tion ssoacie: 0 0 
5) ME 
+ 1%, chlorid of calcium-solu- 
tion: 5 c.c. + water: 5 cc. | 2 6 
6) 2 4 + water: 10 cc. | 1.6 4 
| 
| | 
In the first place it appears from 1) and 3) of this experiment 
‘Table II) that in this case the pancreatic-juice used, contained only 
irypsinogen and no trypsin. Regarded therefore as an investigation 
concerning the activating effect of chlorid of calcium on trypsinogen, 
the experiment could not be seriously found fault with. Except that in 
this experiment no sterile water and sterile CaCl,-solution had been used, 
which evidently should have been done in experiments on the activating 
effect of CaCl, on trypsinogen. I intentionally quote an experiment in 
which no sterile water and no sterile CaCl, were used, as being more 
