( 1009 ) 
by using in the first place a solution of trypsin in 0.1 °, Na, CO,- 
sol. (Table IV A1—6) and secondly a solution of trypsin in a 
2°/, Na Fl-solution (Table IV B 1—6). 
As was observed already the trypsin-GRÜBLER used for this expe- 
riment (Table IV) was the same as that applied in the preceding 
one (Table III); instead of a trypsin-concentration 1/100, 1/50 was 
used. Moreover, as appears from this Table, an intestinal mucous 
membrane extract in water as well as in 2°/ NaFl-sol. was taken. 
It ought to be mentioned that the proper try psinogen-activating effect 
of the extracts of the intestinal mucous membrane was in all cases 
tested by means of pancreatic-juice. 
Peay B EE, IV: 
5 en ee 
‚Digestion of the two 
‚_albumen columns 
| in m.m., after: 
A Ke arn So eee eae: Pee se 3 bee ot 
‚ Trypsin-GRÜBLER | | 
1 gramme to 50 cc. Fluids added 24hrs 48 hrs 72 hrs 
Na,CO3 sol. of 0.1 ®/,; | | | 
1) OG ‚water: 2 cc. 2 | 4g) 35 
2) Sate: | CaCla sol. (10%): 2 cc. 8 16 20 
| 
3). | 30, ‚intest. mucous membrane-extract in 
| 2 0 NaFl-sol. 2 c.c. 4 8 12 
4) STe intest. muc. membr.-extr in water: 2c.c. 3.60 7 10 
5) a) cic: Boiled int. mucous membrane-extract 
in 29/) NaFl-sol.: 2 c.c. 4 8.50) 13 
6) SCE Boiled int. mucous membrane extract 
in water: 2 c.c. Sa Ek oe ED 
Trypsin-GRÜBLER | | 
B \1grammeto50cc.| 
of a 2%, NaFl-sol. _ 
1) Pe OOH | Water:-2.c.c. AE ne PA 8 
2) | 3 cc. | CaClj-sol. (1 %/o): 2 c.c. Ok | Siero 0 
Sul EEA | intest. mucous membrane-extract in 
| 2 0%) NaFl-sol. 2 GE | 4 8 12 
4) 3 cc. intest. muc. membr.-extr. in water: 2c.c. 4.40 850 12 
5) | BI (Ole Boiled int. mucous membrane-extract | 
| in 20/ NaFl sol.: 2 cc. 4 9 13 
6) | & Ce; Boiled int. mucous membrane-extract 
| | in water: 2 cc, 4 S500 412 
