( 1011 ) 
of course, for other salts which may be found in extracts of the intestinal 
mucous membrane, in other words in the intestinal mucous membrane. 
itself, so far as they form insoluble compounds with NaF. 
The results described in connection with the tables inserted, were 
confirmed by other experiments made with the same view, which, 
as was observed already, were carried out with different trypsin- 
preparations of GRÜBLER and of Mrrcx. We subjoin one more experi- 
ment, made with trypsin-Merck. (Table V). 
BABE EV. 
| Digestion of the two 
| albumen-columns 
in m.m. after 
_ Trypsin-MERCK | | 
| gramme to 50 c.c. Fluids added 24 hrs48 hrs 72 hrs 
‚Na2COg3-sol. of 0.1 |, | 
SKA Waters 2 ec. 4 10 11 
SG, CaCla-sol. of 19: 2 c.c. 8 20 empty 
SGC Int. muc. membr. extr. in 2%) NaFl:2c.c. 440 16 empty 
GE: | Int. muc. membr. extr. in water: 2 c.c. 5.00; 15 | empty 
EE Boiled int. muc. membr. extr. in 20/9 
Nas Zec. 5.60 15 empty 
3 Cc. Boiled int. muc. membr. extr. in water: 
De 6 16 ‘empty 
It is seen that the result of this experiment (Table V) is analogous 
to that of the preceding one. Further remarks are not suggested 
by Table V, except that comparison with Table IV, shows that the 
trypsin-Mmrck was somewhat more active than the trypsin-GRÜBLER, 
a fact whieh could invariably be established in the experiments. 
UM MARY. 
1. The experiments described above have shown that chlorid of 
calcium can increase to a considerable extent the activity of trypsin 
which contains no trypsinogen. 
2. This promotive effect of chlorid of calcium on trypsin should 
not be confounded with the activating effect of chiorid of calcium 
on trypsinogen, which latter property is ascribed to this salt by 
several authors. 
3. The extracts of the intestinal mucous membrane were also 
found to possess the property of being able to increase the action 
of trypsin, to a smaller extent, however, than chlorid of calcium. 
