( 1067 ) 
ERAN AET side yh) sll eaten ©, dal, Cue 
DiksttGwmphosphate, %, 2a Sida is 0.05 
Ammonimmneblorid, zn …… 46k) es 0.05 
Calciumchinate (C,H,,O,), Ca + 10H,O O.f to 10 
Perrichlorid wiee la nde Bn GARDE 0.01 
In a wide ERLENMEYER flask, so that a strong aëration oceurs in 
the thin layer, inoculated with soil and cultivated at 20° to 25° or 
30° to 35°C., the liquid colours deep black after a few days, in 
consequence of the formation of ferriprotocatechate. 
To purify the bacterial culture a trace is transferred to a similar 
medium and cultivated at 20° or 30°C, 
If this culture is sown on a medium of the same composition but 
solidified with agar and containing some ferricitrate*), colonies are 
obtained, from the cultures kept at 20° to 25° C., of different 
varieties of B. fluorescens, and from those kept at 30° to 35°C. 
chiefly of a Micrococcus, all lying amid diffusion fields of ferriproto- 
catechate of an intense violet or red colour. This Micrococcus belongs 
perhaps to the same species as that described by EMmmeRriNG and 
ABDERHALDEN, but then certainly to another variety, for it does not 
produce slime, neither in presence of peptone nor of ammonium- 
sait. This form, very common in our environment and whieh can 
be obtained with various other organic salts in a similar way as 
with chinate, I shall name Micrococcus calco-aceticus, as calcium 
acetate is very fit for its accumulation. Here it may be observed 
that acetates are also very useful for the accumulation of certain 
varieties of 5. fluorescens non-lhquefaciens which still grow at 30°C, 
Streaks of these various bacteria on broth agar with one proc. 
caicitum chinate, and a little ferricitrate, or on the above medium, 
give again deep black or red-coloured diffusion fields of ferriproto- 
catechate. 
Part of the chinate oxidises directly to water and caleiumcarbonate 
which precipitates as crystals dyed deeply violet, by having sucked 
up the ferrisalt of the protocatechetic acid during their crystallisation. 
Other species, able to convert the chinic acid into water and 
calciumearbonate or protocatechetic acid, but not found in the fore- 
going experiments, are mentioned in the following table where it is 
indicated by + and — whether the substances placed at the head 
are either or not formed. These experiments were made with broth 
agarplates with 1 proc. ecalciumehinate at 30° C., or use was made 
1) Ferricitrate does not give a precipitate of ferriphosphate in the somewhat 
alkaline broth, 
