( 1068 ) 
of the above named nutrient liquid containing ammonium chlorid, 
after its solidification with agar. 
Protoca- Calcium- 
From chinates result by techetic carbonate as Remarks 
acid crystals 
Bacillus prodigiosus +- 
7 punctatus — — 
Aérobacter coli — — 
5 aérogenes + — Some varieties 
. liquefaciens — — 
Pseudomonas aromatica — = 
fuorescens non 
liquefaciens os Some varieties 
é fiuorescens 
liquefaciens + + Some varieties 
+ pvocvaneus a + 
Proteus vulgaris = — 
Microspira tvrosinatica = — 
Micrococcus calco-aceticus + + All varieties 
Acetic acid bacteria i 
Yeast species = 
This table shows that the common species which oxidise chinate 
to protocatechetic acid, namely the fluorescents, also embrace 
varieties devoid of this faculty. 
The second column is but of relative value, for a number of bacteria 
oxidise the chinate and grow from it with great intensity without 
crystallisation of the thereby formed calciumearbonate. The chinates, 
belong (with the malates) to the most easily assimilable organic salts 
for non-sporulating bacteria in general. 
It is remarkable that there do not seem to exist spore-forming bacteria 
which produce protocatechetic acid, for I did not succeed in obtaining 
microbes from pasteurised materials, such as garden soil or canal 
mud, which, in solutions or on plates of the before given composi- 
tion gave rise to an obvious change of colour. But by various 
spore-formers calciumchinate was changed into carbonate, though 
slowly. 
