( 1148 ) 
Protocol 1, 
June 6th, 710. Str. Dog XVIII. “Dackel”. 
The caudal portion of the thoracal spinal cord is uncovered and 
the dura-mater cleft over Zh. 1X, N and XI, the liquor cerebrospinalis 
that has flown out, is sucked up by a bit of wadding. Then 
strychnin is applied in the following manner : 
A small piece of wadding, long + 3 mm., wound around the 
branches of a thin pair of nippers, is immersed in a 1°/, solution of 
sulfate of strychnine dyed with methylene-blue, and then pressed out. 
With this piece of wadding the dorsal surface of the segment of 
Th.X, at the entrance-point of the right posterior nerve-root, is 
repeatedly bathed with the utmost carefulness. 
After each contact with the strychnine wadding the superfluous 
poison that has eventually been applied, is sucked up by another, 
clean and moist piece of wadding. 
A few seconds after the repeated contact of the poison with the 
spinal cord at this spot, the dog that meanwhile has nearly awakened 
from the narcosis, begins to lick the skin of the right half of the 
trunk over a region, extending like a band of moderate breadth from 
the mid-dorsal to the mid-ventral line, passing over the most caudal ribs. 
The dog itself, with its moist tongue, marks pretty clearly the 
boundary of this zone. The objective part of the syndrome is likewise 
extant and may be easily aroused from out the same skin-zone. The 
hyper-reflectory symptoms are: 1. wrinkling of the skin, 2. curving 
of the vertebral column, the concavity turned to the right, 3. with 
intervals scratching movements of the right hind-leg, resembling 
closely those of the well-known SHERRINGTON's “seratch-reflex”. 
In order to obtain an objective definition of the extension of the 
strychnine-segmental-zone, | touch very gently the skin of the animal 
in the neighbourhood of the region within which the syndrome is 
manifested, either with one of my finger-tips, or with the point 
of a curved; shut pair of nippers. 
Continually repeating this gentle, mechanical irritation, [ gradually 
approach that region, and as soon as I have passed its boundary, 
the hyper-reflectory symptoms described above are aroused or become 
much more intense, whilst in most cases') the animal shows at the 
1) [ say “in most cases’, because the intensily of the subjective part of the 
syndrome presents great variations in different cases, partly dependent on “tempe- 
rament and character” of the animal, whilst for another part they may perhaps 
be ascribed to its more or less complete awakening from the narcosis. 
