( 1209 ) 
Katalase, produced by the bacteria, plays no part therein, neither 
could this be shown for oxidases, these enzymes exerting no action 
on the fat-layer in the gelatin. 
The favourable influence of light on the decomposition of fat in 
presence of lipase may be demonstrated by exposing some fatted 
tubes containing lipase gelatin to the light and compare them with 
similar tubes kept dark, whilst for control, tubes without lipase were 
exposed to the light and others kept out of it. 
In the tubes containing gelatine with lipase and exposed to the 
light the fat is more strongly affected than in those prevented from it; 
the tubes acted upon by light and oxygen are most vigorously attacked. 
In the tubes containing gelatin without lipase a slight decomposition 
of the fat is observed but it is inferior to that of the tubes with 
gelatin and lipase. 
Synthesis of fat from glycerine and fatty acid by microbic lipase. 
This lipase, as we have seen, splits fat in glycerine and fatty acid 
in presence of much water; it is able from glycerine and fatty acid 
again to build up fat, if very little water is present. 
Some material of B. lipolyticum @ was used as a lipase preparation. 
These bacteria had been cultivated on broth-agar and after 10 
days at 30°C. been taken off the agar with a spatule. 
In this way about four and a half ¢.c. material had been procured. 
This lipase preparation was introduced into a stoppered bottle 
with = 50 c.c. oleic acid and = 60 ce. glycerine (S. W. 1.25), 
and after addition of a few glass beads for better mixing, the bottle was 
filled with glycerine, for which about 25 c¢.c. sufficed. 
After the contents of the bottle had been rendered as homogeneous 
as possible by vivid shaking, 5 ¢.c. were withdrawn from it and 
supplied by 5 e.c. glycerine. These 5 ¢.c. oleic acid + glycerine 
titrated 77.2 c.c. */,, N. caustic soda. 
The bottle was now heated to 40° and remained 48 hours 
at this temperature, being during that time repeatedly and _ vividly 
shaken. 
5 ee. of the contents of the bottle then titrated 56.2 cc. 1/,, 
N caustic soda so that about '/, of the oleic acid had disappeared. 
The liquid was subsequently poured into two L. of boiling water 
and shaken with it, whereupon the oily layer was separated from 
it. The oily liquid was washed with hot water and shaken with a 
*/,°/, natriumcarbonate solution by which the still present oleic 
acid dissolved into natriumoleate. 
