( 1210 ) 
The remaining liquid was mixed with calcinmearbonate, heated 
to + 200° C., then sucked off. 
After this treatment 11.5 c.c. liquid was obtained of an oily 
consistency and yellow colour. It reacted neutrally as to litmus and 
was soluble in ether, benzene and carbon disulphide. 
By heating with alcoholic kali saponification took place. A gram 
of this compound uses 163 mG. of KOH. 
The S.W. amounted to 0.958. 
Evidently in this synthesis the monoglyceride of oleic acid is 
chiefly formed, as follows from the S.W. and the saponification 
number; at the same time slight quantities of di- and tri-glycerides 
are present. 
Summary. 
1. The composition of the medium is of no consequence for the 
secretion of lipas: by microbes; so that every source of carbon or 
nitrogen, assimilated by a fat-splitting organism, will serve equally 
well for the production of lipase by this organism. 
2. The secretion of acids by microbes in a culture medium 
diminishes the secretion of lipase. 
3. Acids form compounds with lipase from which lipase is again 
freed by bases. The acid-lipases diffuse like lipase through gelatin 
and agar culture media; acid-lipases of higher fatty acids do not 
diffuse; acid-lipases do not decompose fat. 
4. Hydrogenions retard, hydroxylions accelerate the action of 
lipase. If the acid degree of a medium exceeds '/,, N no more fat- 
splitting by lipase takes place. Lipase behaves to acids as a feeble 
basis. 
5. Calcium- and magnesium-ions favour the action of lipase; 
likewise trimethylamine and natriumglycocholate; monovalent alcohols 
counteract the process, sugars and glycerine exerting no influence. 
6. Presence of oxygen and light favour the decomposition of fat 
by the action of lipase. 
7. By means of microbic lipase fat may be synthetically obtained. 
From oleic acid and glycerine chiefly the monoglyceride results but 
besides, probably a little di- and triglyceride. 
8. Microbic lipase shows great similarity to liver- and pancreat.e 
lipase. 
