794 
showed a distinctly immunizing action, but the serum of goat B 
a twice as great action as the serum of goat A (see experiment n° 2). 
Comparing the sera got 31 days after the injection, the immuni- 
zing action of the serum of goat B still proved to surpass that of 
voat A distinetly. 
It would have been clearer, if we had been able to inject twice 
as much serum of goat A as of goat B. But in the mean 
time the immunizing action of the serum of goat A had declined 
in such a way, that the quantity to be inoculated became too large 
to be injected into mice (see experim. N°. 3). 
EA PER 1 EON TT NGS: 
Go alte A: Gorab Be 
Mouse Date Subcut. Result Mouse Date Subcut. Result 
N°. 438 25 Sept. 2.0c.c. serum N°. 439 25 Sept. 20c.c. serum 
Npe 0.3 c.c. toxine Or 0.3c.c. toxine 
ti local tetanus PM pee no symptoms 
Be. is general „ ‘ae no symptoms 
a dead 29 „ no symptoms 
A0 30, no symptoms 
‘mmm re 
CONTROL 
Mouse Date | Subcut. Result 
N°. 440 26 Sept. 03c.c. toxine 
DT 5 local tetanus 
Zo dying 
20 dead 
As we had to consider the possibility that the immunity of goat 
B did last longer because this animal was less fit to produce anti- 
bodies against horse albumen than goat A, we have repeaied the 
experiment on two other goats. 
We chose again two goats of about the same age and size, and 
injected subeutaneously into goat C 80 A.U. in 20 ¢.c. serum, into 
goat D 80 A.U. in 40 cc. serum. At the 10%, 17%, 24th and 31* 
