809 
nearly 25°/, of the total, would, with C, have a narrow red leaf 
margin, and this phaenotype is absent. 
The total absence of plants which do not at the same. time have 
both the factors A and b, but have C and ought to be able to 
show that factor separately also excludes any explanation based on 
imperfect coupling or repulsion of C with vespect to A and B. 
Three possibilities still remain: «. part of the germ cells die off; 
b. a factor might come into play which prevented the manifestation 
of C and was itself dependent on the presence of A and Bb; ec. with 
complete coupling of C to A and B unilateral reduplication might 
occur, as HERIBERT-NILSSON *) postulates in some cases for the factor 
for red leaf veins in Oenothera Lamarckiana. Such reduplication 
would then occur, not, as is supposed in Oenothera, in the germ 
cells which possess this factor, but rather in those which are deficient 
in the factor U. 
Of course the F2 must be crossed back with the recessive form, 
for this character therefore with C. glauca, and this will take much 
time. Moreover the same variability in the segregation ratios may 
be expected as in self-pollination, so that the ‘question will probably 
not be cleared up much by this. 
That the confusion of the Mendelian segregation involving a large 
number of factors need have no permanent effect on the offspring, 
is shown by the ratios of some of the F3 numbers. 
TABLE Il. Segregation in F3 for the character of red leaf margin. 
Narmiaer |: Number of | With: red Ratio 
0 | 2 
F2 N°. of seeds. seedlings | margin Green red: green Theory 
| 
1 10 48 36 12 Stal 36: 12 
9 67 49 28 21 Ont 27,6 : 21,4 
Wax on the leaves. 
Whether a definite wax tayer is present on the leaves, as in 
C. glauca, or whether it is absent, cannot be determined in young 
seedlings. Not until 1—1'/, months after planting out does the chance 
of error become small, but even then doubtful individuals remain, 
which are best judged by subsequently formed shoots. 
The number of factors for wax is still unknown: it wil! most 
likely become evident in F3, but then only for these numbers of 
1) Hertpert-Nitsson, N., Die Spaltungserscheinungen der Oenothera Lamarckiana, 
1916. 
