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5°/,, vuzinal bihydrochloridum to 1°/, (5°/, solutions are clear again, 
concentrations between these values are turbid). In a physiological 
common-salt solution, Ringer-, or Tyrode-solution, turbidity practically 
exists in every concentration. 
In serum eukupin-biHCl dissolves to 1:14000, vuzin-biHCI 
to 1: 20000. When the solutions in serum are made to foam, the 
two substances are collected in a higher concentration in the foam 
than in the liquid. The foaming is diminished by the addition of 
much alkaloid-salt. 
3. With subcutaneous injection the fatal dose for white mice per 
ke. body-weight is for eukupin: 300 mgr. and for vuzin: 200 mgr. 
So the toxicity of either substance, administered subeutaneously, is 
for mice two- or three-times greater than that of quinine. 
The subcutaneous fatal dose for cats per kg. body weight, amounts 
to from 25 to 50 mgr. of eukupin, 200 mgr. of vuzin. 
4. With slow intravenous injection the fatal dosage per kg. cat 
varies with the concentration of the alkaloid salt: in a 1°/, solution 
it amounts per kg. cat to about 13 mer. of eukupin and about 
15 mgr. of vuzin; in 1°/,, solution per kg. cat to 70 mer. of eukupin 
(in one experiment, in which vagi intact); and 40-—120 mgr. of 
vuzin (vagi intact or cut). 
In the case of rabbits the intravenous fatal dosis of eukupin (in 
1*/,,-solution) seemed to vary with the Nn.-vagi being unimpaired 
or cut through: it was per kg. rabbit with unimpaired vagi about 
13 mgr, with vagi cut about 60 mgr. It appears from this that in the 
rabbit eukupin acts upon the vagus-center. 
5. After subcutaneous injection of eukupin and vuzin cats die 
under a progressively increasing sopor. Large doses of eukupin cause 
a marked fall of temperature. 
6. Subcutaneous injection of concentrated solutions (5°/,) of the 
two alkaloid-salts brings about local necrosis of the skin and the 
subcutaneous connective tissue. 
7. Cleansed sheep’s blood-corpuseles suspended in Ringer’s solution, 
were hemolyzed through eukupin in a concentration of about 1 : 5000 
through vuzin in a concentration of about 1 : 10000. 
The number of red blood-corpuscles per mm* plays some influence 
upon the required concentration of the alkoloidsalts. 
In the presence of serum the concentration of both substances, 
required for hemolysis, is about 1 : 1000. 
8. Kukupin and Vuzin in 1 °/,-solution convert oxyhemoglobin 
into a brown colouring substance, which in an acid as well as in 
an alkalin solution shows in the absorption-spectrum spectroscopically 
