493 
described in the preceding communication for the adsorption of cocain 
by serum *). The alcohol was evaporated, the liquid neutralized and 
ty 
4 a | Oot mig A. 
/N 
LN en . ; 
‘Ae SOA mgr. 
AALLALLI PIYRUTSNIVOTUUUTEDOUOVIDO VOLES Dec oecccloee “OT TTT 
he JS Aum at of Ona Op PS ip 
mel he acther feex EE se 
Fig. Ig, Ih. 
made up to the original volume. Now we ascertained how much 
active atropin was present. The result can be seen in fig. 1 f—1h 
In fig. 1f and 1h every time 0.2 mgr. of pilocarpin is added to 
the gut and afterwards 0,0006 mgr. of atropin’). As will be seen 
from the figure after three minutes nearly the whole action of the 
pilocarpin has been abolished by the atropin. In fig. 1g again 0,2 
mgr. of pilocarpin is given, and after three minutes so much of the 
_atropin-containing liquid (serum + atropin treated with alcohol and 
hydrochloric acid) has been added, as corresponds to 0,001 mgr. 
atropin of the original serum-atropin mixture. It will be seen that 
its action is slightly stronger than that of 0,0006 mgr. of atropin, 
from which we deduce that through the treatment with alcohol 
if not all, yet nearly all the atropin has been recovered in full 
activity. 
1) L. EERLAND and W. STORM VAN LEEUWEN. l.c. 
2) The piece of gut used here was not the same as that of fig. la—e. 
32 
Proceedings Royal Acad. Amsterdam. Vol. XXIII. 
