497 
It would be well to observe the quantitative values of adsorption 
for a large number of concentrations of atropin in rabbit’s serum; 
this however would be a difficult task also on account of the time- 
consuming technique; anyhow from the single experiment just de- 
scribed (similar experiments led to the same results) it appears that 
probably the relations obtained with the adsorption of pilocarpin by 
rabbit’s serum also hold good for the adsorption of atropin by serum; 
the said relations have been discussed by one of us in an earlier 
communication. *) 
It was there that we found nearly all the poison adsorbed when 
working with comparatively small quanta of the alkaloid; larger 
quantities produced a different effect. 
If, namely, in that experiment 10 mgr. of pilocarpin was added 
to 5 ec. of rabbit's serum, 9.5 mgr. were adsorbed, so that only 
0.5 mgr. was left in the solution; an addition of 20 mgr. of pilo- 
carpin yielded an adsorption of 18,5 mgr. of pilocarpin, so that 
only 1,25 mgr. remained. Larger doses however gave widely different 
results: e.g. an addition of 100 mgr. led to an adsorption of 28,5 mer., 
so that 72,5 mgr. was left in the solution. As said, such an extensive 
quantitative inquiry in this direction fur atropin, takes a great 
deal of time and is almost impracticable. The experiment recorded 
here, however, points to the probability of similar quantitative rela- 
tions for atropin to those for pilocarpin. 
In conducting these experiments we observed something that 
necessitated an extension of our investigation. We found that the 
serum of a rabbit, to which before death an injection of some c.c. 
of a peptone solution had been administered, had no or only little 
adsorbent power. It also appeared that rabbit’s blood, to which citras 
natricus had been added to prevent clotting, had only a very slight 
adsorbing power for atropin. 
We might reasonably infer from this that the substance from 
rabbit's serum which can adsorb atropin, is not present as such, but 
is generated only after clotting, so that no adsorption will take 
_ place when coagulation is prevented. In looking through the literature 
this supposition appeared to be improbable, because Mrrzner’) made 
his experiments with rabbit's blood to which hirudin had been added, 
and he does not mention that this addition lessens the adsorbent power. 
The supposition is also refuted by Dovon and Sarvonat*), who 
1) W. Storm van LEEUWEN. |. c. 
ke. 
5) Doyon et SARVONAT. Passage d'une nucléoproteide anticoagulante dans le 
sang. Soc. de Biol. 74. 1913, p. 78. 
