5 



Injection of secretin in most cases lowers the amount of sugar in 

 the blood, the average tall being 80 7o- This faculty of secretin 

 asserts itself even in counteraction to suprarenin, which of itself 

 produces an increment of 100 7o ^t the end of Vl^ hours, but only 

 50 7o under the given circumstances. 



However, glucosuria appeared in all cases but one. That llie 

 results communicated here are due to the solo action of secretin lias 

 been distinctly shown by the experiments, which yielded negative 

 results with injection of other intestinal extracts. 



These experimental investigations led me to endeavour (o tind out 

 whether an internal secretum of the pancreas could actually be 

 elicited and whetiier injection of secretin excited its activity. 



Seco7id series (B). 



It has often struck me, that only few workers have strenuously 

 exerted themselves to obtain in vivo the internal secretum of the 

 pancreas, putting aside of course Biedl's ^) and de Mp:yer's ^) experi- 

 mentation. The former examined the anti-glucosurian action of the 

 lymph from the thoracic duct. The latter the effect of pancreatic 

 extracts or of fluids which had been made to pass through the 

 pancreas artificially, on the glycogenesis of the liver. These researches 

 constitute the best work that has been done in this field, as (he 

 other investigations were restricted to biological experiments with 

 blood from the pancreatic vein, especially as regards its fermentative 

 properties. 



Our purpose was ratlier to furnish large quantities of blood from 

 the pancreatic-duodenal vein for further experimentation on carbo- 

 hydrate metabolism. Let it be stated beforehand, that in large dogs 

 such quantities are not difficult to procure from this vein. 



Method. In large dogs of 10 — 15 K.G. an incision is made 

 through the abdominal wall parallel to the arcus costalis to get 

 access to the pancreas, which is drawn out by the hook-shaped 

 finger and immediately wrapped in cloths soaked with a warm 

 physiological common salt solution. 



It is then easy to tind the pancreatic-duodenal vein, which is 

 ligatured as near the trunk of the portal vein as possible. Subse- 

 quently a cannula is inserted, which cannot always be managed. In 

 case of failure the vein is simply cut through. At the same time 



1) BiEDL. Wiener Kl. Wochensch. 1907. 



"l ÜE Meyee. Archiv. Intern, de Phyü. 1910. 



