6 



20 — 40 cc. of secretin is injected intravenously or subcutaneously. 

 Most times ttie blood is discharged rapidly, sometimes it issues in 

 a jel; the blood flow can still be accelerated by kneading the pan- 

 creas with the full hand and by massage. In this way 80 — 250 cc. 

 of blood can be collected in about V4 hour. In some of our experi- 

 ments the blood was allowed to coagulate and we used the serum 

 separated in the clotting. Most often, however the blood was defi- 

 brinated at once. 



With this blood or with this serum the following experiments 

 were performed : 



Specimens of this blood varying from 5— 20 cc. were used upon 

 rabbits^) subcutaneously, in which also V» nig'"- of suprarenin ^) was 

 injected. After the lapse of IV2 hours the blood-sugar percentage was 

 determined. The experiments however varied in some details according 

 to the various treatment of the pancreas blood that had been admin- 

 istered. Usually three specimens of dog's blood were given separately 

 to three rabbits of equal weight. The specimens, however, were all 

 different inasmuch as either the blood had flowed from the vein 

 prior to the injection of secretin, or this had occurred concomitantly 

 with the injection, or the blood had been heated for halfanhourat 

 56° C, or finally the blood had been liberated from its proteins by 

 addition of three times its volume of alcohol, the alcoholic filtrate 

 having been evaporated down to its original volume. 



We subjoin a table illustrating the results of some of our most 

 successful experiments. 



By the side of these experiments others were made upon rabbits 

 with homologous serum. It being, however, very difficult to draw 

 an adequate quantity of blood from the rabbit's pancreatic vein, we 

 have to content ourselves with reporting only one satisfactory test. 

 From the pancreatic vein of a rabbit, weighing 3 K.G., 8 cc. of 

 blood was obtained. Four cc. of serum were separated . Subsequently 

 12 cc. of secretin were injected per vena auricularis; and 15 cc. of 

 blood, furnishing 7 cc. of serum, were drawn from the pancreatic vein. 



Next day 2 rabbits, weighing 1300 grras were injected intravenously. 

 The first got the 4 cc. of serum of the first sample and immediately 

 after 1 mgr. of levorotatory suprarenin. The percentage of sugar in 

 the blood was 0.297 after r/a hours. The urine passed in 24 hrs. 

 was 15 cc. with 0,6 Vo of sugar. 



1) In order to avoid complications the foreign serum was injected only once 

 into each rabbit. 



2) lu this experiment as in all others only fresh suprarenin was used from 

 vials that had just been opened. 



