22 



Thereby the serum woiikl be more diluted than could be calculated 

 from the addition of the solution which contained no chlorine. That 

 in spite of this the chlorine of the serum agrees with the value 

 calculated beforehand, proves that, with the water, chlorine has 

 passed from the blood-corpuscles into the serum. 



The following experiment may serve as an example : 



EXAMPLE. 



Change in the amount of chlorine of the serum of horse's, cow's, and dog's 



blood, if 12 0,0 of the serum has been replaced by sulphate-solution. 



It was, therefore, certain that blood-corpuscles with a normal 

 permeability always react in the same way if part of the serum is 

 replaced by a Na^ SO 4 -solution. 



When chloroform was added to the blood — so much even that 

 slight, but distinctly visible haemolysis set in - — the same rules 

 weve found to hold good: the transfer of chlorine under the influence 

 of Na^SO^ is the same in normal and in chloroform-blood. 



EXAMPLE. 



The permeability of the blood-corpuscles is determined before and after 



l.20|^JQ of chloroform has been added to the blood. 



Of 15 cc. of blood 1 cc. of serum is replaced every time by: 



1 cc. of H.O. 



The chlorine of 5 cc. 



of serum corresponds 



with : 



1 cc. of 2 X hypertonic 



Na2S04-solution. 



The chlorine of 5 cc. 



of serum corresponds 



with : 



1 cc. of isotonic 



Na2S04-solution. 



The chlorine of 5 cc. 



of serum corresponds 



with : 



normal 

 blood 



5.13 cc. 

 AgNOa 



chloroform- 

 blood 



5.12 cc. 

 AgNOa 



normal 

 blood 



4.93 cc. 

 AgNOa 



chloroform- 

 blood 



4.96 cc. 

 AgNOa 



normal 

 blood 



4.94 cc. 



AgNOg 



chloroform- 

 blood 



4.96 cc. 

 AgNOa 



