23 



Hence the conclusion was conji fined that an addition of chloroform 

 does not aj/'ect the pernieabilitij of the red blood-corpuscles. 



Does the fact that lipolytics do not affect tlie permeabiJity of the 

 red blood-corpuscles, prove that their i)ernieability cannot be 

 modified? 



Others have proved from analyses that the view that the stroma 

 of the red blood-corpuscles consists exclusively of lipoids, is incorrect. 

 Only a third part of the stroraata are formed by lipoids, the other 

 two thirds are albuminous substances ^). 



For the permeability for anorganic substances, however, the subject 

 under consideration, the albuminous part of the stroma of the 

 blood-corpuscles will be the most impoitant: lipoid membranes are 

 impermeable for anorganic substances, albuminous membranes are 

 permeable. A priori it is, therefore, probable that lipolytic substances 

 will not affect the permeability for anorganic matter, as they leave 

 the albuminous part of the stroma intact. 



In order to settle the question about a change in the permeability, 

 another substance than chloroform had to be added to the blood. 

 An acid was taken, because acids act upon albumin. 



It was known that acid, added to blood, causes the blood-corpuscles 

 to swell and effects a transfer of chlorine from the serum to the 

 blood-corpuscles. This also takes place when acid is added to a 

 suspension of other cells in serum '■'). 



This transfer of water and chlorine was observed again when 

 these experiments were repeated. It was, however, also observed 

 that after an acid had been added, the permeability of the red 

 blood-corpuscles had changed. The latter conclusion was based on 

 the following observations : 



1. Of 15 cc. of blood 1 cc. of serum was replaced by 1 cc. of 

 an isotonic Na.SO^-solution, to which a trace of H^SO^ had been 

 added. The transfer of chlorine, taking place now, is considerably 

 greater than that effected by H^SO, only. In normal blood an 

 addition of the same volume of Na,S04 causes no transfer of chlorine. 

 If, however, an acid has been added to the blood, the Na^SO^ 

 increases the ti-ansfer of chlorine, under the influence of the acid. 



Hence Na^SO^ acts in, different manners upon normal blood and 

 upon blood to lohicli an acid has been added : the permeability of 

 the red blood-corpuscles has been affected by the acid. 



1) Pascucci. Hofmeister's Beitrage. Bd. VI. 1905. 



2) Hamburger I.e. 



