24 



EXAMPLE. 



In normal blood hardly any transfer of chlorine if + 12% of the serum is replaced 



by 2 X hyperton. Na2S04 solution. 



In blood to which an acid has been added, a considerable transfer 



of chlorine, under the same circumstances. 



Of 15 cc. of blood 1 cc. of serum is replaced every time by: 



1 c.m3. - n . H2SO4 



1 cc. of a ^ n . H2S04-solution, 



which at the same time contained so 

 much Na2S04 that it was 2Xhypertonic: 



chlorine of 

 4 c.m3, serum 



4.23 C.m3. AgNOa 



chlorine of 4 cc. of serum 



3.96 C.M3. AgNOa 



2. As we said before, in normal blood it makes no difference if 

 of 15 cc. of blood 1 cc, of serum is replaced by 1 cc. of isotonic or 

 1 cc. of 2 X hypertonic Na^SO^-solntion. In blood to which a trace 

 of an acid has been added, these values are different. An addition 

 of 1 cc. of 2 X hypertonic Na^SO^-solution causes much more chlorine 

 to enter the blood-corpuscles than an addition of 1 cc. of isotonic 

 Na^SO^-solution.. 



This furnishes another proof of the changed permeability of the 

 blood-corpuscles. 



EXAMPLE. 



In normal blood no difference between the transfer of chlorine, caused 



by an isotonic Na2S04-solution, and that, caused by a 



2 X hypertonic Na2S04-solution. 



In blood to which at the same time an acid has been added, a difference is found. 



Of 15 cc. of blood 1 cc. of serum is replaced every time by 



1 cc.of 2 X hyper- 

 tonic Na2S04- 

 solution (7. 40/0) 



1 cc. isotonic 

 Nac>S04-solution 

 " (3.1%) 



1 cc. of a 1 



n . H^S04-solution 



containing also 



7.40/0 Na2S04 



1 cc. of a 



20 



n . H2S04-solution 

 containing also 

 3.70/0 Na2S04 



4.15 cc. of AgNOa 



4.18 cc. of AgNOa 3.96 cc. of AgNOa 



4.08 cc. of AgNOa 



chlorine of 4 cc. serum. 



This proved that it was possible to modify the permeability of the 

 blood-corpuscles by the addition of acids. 



