25 



II. Interchange of chlorine betioeen hlood-corpuscles ami 

 medium in human blood. 



It had to be investigated now to what extent these facts could 

 contribute towards an explanation of the chlorine-retention in febrile 

 diseases. 



For this purpose the permeability of blood-corpuscles, of persons 

 with a normal chlorine excretion was first examined. The results 

 were as follows : 



1. If in 10 cc. of human blood 0.6 cc. of serum is replaced by 

 0.6 cc. of an isotonic NajSO< solution, the amount of chlorine in the 

 serum is greater than the dilution vvith the solution containing no 

 chlorine, would lead us to expect. 



Under these circumstances, therefore, chlorine passes from the 

 blood-corpuscles into the serum. Evidently human blood-corpuscles 

 behave in a manner different from horse's, cow's, or dog's blood- 

 corpuscles if the medium in which they are suspended, is modified. 



2. If to 10 cc of human blood 25 mgr. of crystallized Na^SO^ 

 is added, chlorine passes from the blood-corpuscles into the serum. 



EXAMPLE. 



Transfer of chlorine in human blood if + 12 \ serum is replaced by isotonic 



Na2S04 solution, or if crystallized Na2S04 is added to the blood. 



Chlorine in4cc 



of normal serum 



corresponds 



with : 



lOccof 



blood 



contain: 



After 25 mgr. of 

 Na2S04 have been 

 added to 10 cc of 

 blood the chlorine 

 in 4 cc of serum 

 corresponds with : 



Of 10 cc of blood 

 0.6 cc of serum is 

 replaced by 0.6 of 

 isotonic Na2S04 

 solution. 



The chorine in 

 4 cc of serum 

 corresponds with : 



If no transposition 

 of chlorine had 

 taken place, the 

 chlorine in 4 ccof 

 serum would in 

 the latter case have 

 corresponded with: 



4.24ccofAgN03 



6.8 cc of 

 serum 



4.44 cc of AgNOa 



4.05 cc of AgNOa 



6.8-0.6 



X4.24 = 



6.8 

 3.85 cc of AgNOg. 



In a series of cases, in which the blood was examined of people 

 in good health and of patients, of people whose food contained much 

 and of others whose food contained little chorine, of people with 

 and without fever, these two results were invariably arrived at. In 

 all these cases, however, the metabolism of chlorine was normal. 



The same experiments were now carried out with the blood 

 of fever patients showing marked retention of chlorine. The results 

 were as follows ; 



