2.50 



stances this was not done in one case, and in another the meal 

 was given onlj 5 hours prior to the operation. 



The amount of glycogen was estimated after Pflüger's method ; 

 immediately after the extirpation the portions of liver were put in 

 a 60 7„ solution of KOH and were weighed. 



The detailed reports of the experiments contain particulars relative 

 to the fluids injected intravenously. 



FOURTH SERIES (Ü). 



1. On the 24th of December 1912 I extirpated 38 grs. of the liver of a dog, 

 weighing SVa KG , which had been fed with amylacea eight hours before. The 

 piece of liver was forthwith subjected to the process described. Hereafter I injected 

 per pancreatic duodenal vein 50 c c. of a mixture of 100 cc. of serum + 50 cc. 

 of defibrinated Ijlood + 50 cc. of Locke's solution. 



The remaining 150 cc. of this mixture was injected slowly per jugular vein. 

 After an hour's irrigation another j)Ortion of liver of 24 grs. was removed and 

 subjected to the same process. 



The blood and the serum had been obtained the evening before from the pan- 

 creatic vein of a dog under the influence of a subcutaneous injection of 50 cc. 

 of secretin. The blood had been kept cool, not in the refrigerator. 



The glycogen of the 1st portion of liver amounted to 3.825 ^/q. 

 „ , 2d , „ , , „ 3.905 0/0. 



So there was a slight increase of glycogen of 3 ^/o- 



2. On the 28th of December 1912 26 grs. of liver were taken from a dog of 

 12 KG. Directly after this 180 cc. of Locke's solution was given per jugular vein 

 from a burette in the space of two hours. The laparotomy-wound was covered 

 with warm tampons. At the end of the experiment the rectal temperature was 

 36.7°. The solution had been heated to 30^ G., the room-temperature was 

 20 — ^22° G. After the irrigation a second portion of the liver (29 grs.) was excised ■ 



The amount of glycogen of the 1st portion was 1.71 ^Jq. 

 . „ „ „ „ 2d „ , 0.895 o/„. 



This reveals a considerable loss of glycogen of 50 o/q. 



3. On the 30th of December 1912 a dog weighing 15 KG. was irrigated in a 

 similar manner with 180 cc. of Locke's solution during one hour and a half. 

 Beforehand 41 grs. of liver had been extirpated and again 37 grs. after one hour 

 and a half. 



The amount of glycogen of the 1st portion was 3.995 o/p. 

 „ , . „ „ 2d , , 1.712 0/0. 



Consequently a loss of 56 o/^. 



4. A dog of 8 KG., which had not had the ordinary diet, was irrigated intravenously 

 with the following mixture : 60 cc. of defibrinated blood + 40 cc. of serum, ob- 

 tained from jugular blood collected the day before from a healthy dog. To this 

 mixture was added 50 cc. of Locke's solution. The irrigation lasted one hour and 

 a half Beforehand a portion of the liver (31 grs.) had been removed. After the 

 irrigation a piece of 34 grs. 



