251 



Tlie amoLinl of glycogen of llie 1^1 piece was 0.l7l"/()- 



Sc the loss was 36 "^/o. 



5. On the Glli of January 1913 llie following mixture was thrown into a dog 

 of 9 KG. per jugular vein during one liour and a. half: 88 cc. of blood + serum 

 (obtained from the pancreatic duodenal vein under the influence of secretin) 

 + 70 cc. of Locke's solution Room-temperature somewhat lower than usual (20° C). 

 The abdomen was covered with warm cloths soaked with a physiological salt- 

 solution. 



39 grs. of liver had been removed before the process, 30.8 grs. after it. 



The amount of glycogen of the first portion was 4.23 ^/q. 

 „ . second , „ 4.17 "/q. 



In neutralizing portion 1 after the inversion' a slight excess of KOH was added 

 somewhat too quickly, which originated a light brown decoloration. 



G. On the 19^'» of January 1913 a dog of 11 KG. was given per jugular vein 

 during one hour and a half a mixture of 100 cc. of serum + blood + 40 cc. 

 of Locke's solution. The blood had been obtained the previous evening from the 

 pancreatic vein under the influence of secretin. The dog from which it had been 

 drawn was very old. The pancreas had greyish white spots, rather smaller than 

 a threepence : there was besides suppuration of the cysts in the postate ; the urine, 

 however, contained no trace of sugar whatever. 



After the first extirpat'on of liver venous hemorrhage ensued, which could be 

 staunched only by ligation. The room-temperature was 19° G. 



Previously 26.6 grs of liver had been excised. After one hour and a half 29 grs. 



The amount of glycogen before the irrigation was 3.61 %. 

 after „ „ „ 2.87 o/o- 



This points to a loss of 21 %. 



7. On the 25''^ of January 1913 the following mixture was used per jugular 

 vein upon a dog of 5.5 KG. : 60 cc. of defibrinated blood -f 30 cc. of serum from 

 the jugular vein of a normal dog -|- 50 cc. of Locke's solution. Room-temperature 

 26°— 22° G. Duration of the test one hour and a half. 



32 grs. of liver had been removed previous to the irrigation, 29.7 grs. after it. 

 Amount of glycogen before the irrigation 2.638 %. 

 after , , 1.677 0/,. 



Loss 33%. 



8. On the 28"' of January 1913 24.8 grs. of the liver of a dog (6 KG.) was 

 excised. Injection per jugular vein of: 25 cc. of defibrinated blood + 40 cc. of 

 serum from the pancreatic vein (obtained the evening before) taken up with 20 cc. 

 of Locke's solution are freed from protein by three times their quantity of 96 "/q 

 alcohol. After filtration the mixture is evaporated down to 80 cc. at 70° G. and 

 again Locke's solution is added to 150 cc. A filtration error causes a loss of 50 cc. 

 so that an equivalent volume of Locke's solution has to be added again. 



24.8 grs. of liver had previously been removed and 23.8 grs. after one hour 

 and a half. 



Amount of glycogen before irrigation 2.024 %. 

 n n n after , 1.03 Vo- 



Loss 50 o/q. 



