252 



During the irrigation llie clog had two syncopes so tliat tlie respiration had to 

 be maintained artificially. 



9. On the 31^' of January 1913 a dog of 7 KG. was given 200 cc. of a mixture 

 of 50 cc. of blood -j- 100 cc. of serum obtained the previous evening under the 

 influence of secretin from the pancreatic vein of a dog. Locke's solution was added 

 to 200 cc. Besides a secretin injection this dog had also had a light meal of 

 carbohydrates. 



The irrigation lasted one hour and a half; only once breathing came to a 

 standstill. 



Beforehand 25.1 grs. of the liver had been excised, afterwards 28.4 grs. 

 Amount of glycogen of the 1^' portion was 2.36 ^ q- 



2"d 1 77 ^L 



Loss 20 0/ü- 



10. The same large dog from which pancreatic blood had been drawn for 

 injection of the dog in N^. 9, had the pancreas removed. Part of this (8/5) was rubbed 

 up with about 40 cc. of Locke's solution and mixed with three times this quantity 

 of 96 7o alcohol. After filtration this liquid was evaporated down to Vs of its 

 volume in a porcelain dish at 70° G. and subsequently Locke's solution was added 

 to 180 cc. 



Three days after the preparation (on the 3'"^ of February) the liquid was thrown 

 into a dog of 4 KG. 



Previously 1?.2 grs. of liver had been extirpated. 12.4 grs. one and a half hour 

 later. 



The amount of glycogen of the first portion was 2.41 ^'/o- 



» » second „ „ 1-48 %. 



The loss, therefore, appeared to be 37 ^\^. 



11. On the 11"^ of February 1913 a dog of 12 KG. was given during one hour 

 and a half per jugular vein normal blood (100 cc. made up to 140 cc. with Locke). 

 First I tried to inject part of it per pancreatic vein. However, when 10 cc. had 

 been injected a venous hemorrhage had to be staunched. We therefore performed 

 further injections per jugularis. 



Four hours before the dog had taken a copious meal of amylacea so that the 

 digestive process was at its height. The narcosis proceeded regularly. 



Of the liver 14.6 grs had been removed before the injection and 40 grs an 

 hour and a half after it. 



Amount of glycogen of the first portion 7.823 "/o- 

 „ „ second , 6.433 "/j. 



Here the loss was 18*'/o. 



12. An extract of the pancreas of the dog whose normal blood had been used 

 in N". 1] was used upon a dog of 5 KG. The extract had been prepared as 

 follows: The pancreas, weighing 23 grs, was rubbed up with Locke, then heated 

 for one hour and a half to 60° G. to destroy the ferments; after this it was 

 subjected to a low temperature (about 5° G.) and used next day after filtration through 

 cotton wool and addition to 190 cc. After precisely an hour's irrigation a shock 

 occurred; in less than no time a portion of the liver was cut off. 



Extirpation of 14.6 grs of liver before injection; after an hour 13.3 grs. 

 Amount of glycogen of the 1^^ portion 10.88 %. 

 , . . . n 2"<i , 6.76 0/,. 



Loss after an hour 38 ^/q. 



