253 



13. On the 25*ii of February a mixture of secretin and Locke's solution was 

 thrown into the jugular vein of a dog weighing 7 KG. The secretin had been 

 prepared as follows: In the space of 2 X '^ liouis an extract was made of tlie 

 mucous membrane of the duodenum with absolule alcohol in Soxhlet's apparatus; 

 only then it was macerated with HCl and after boiling up neutralized with sodium. 

 There was a secretin solution of 50 cc. to which 130 cc. of Locke's solution had 

 been added. Irrigation for one hour and a half. 



Of the liver 24 grs had been excised before the injection and 88 grs one hour 

 and a half after it. 



Amount of glycogen of the 1** portion 4.356 '■%. 



o ml 0) PXin 0/ 



Loss of glycogen 87 o/,,. 



14. On the 3'^ of March a dog of G KG. was given per jugular vein 50 cc. of 

 pancreas blood + 60 cc. of serum -|- 50 cc. of Locke's solution, obtained the 

 preceding evening from a dog under the influence of secretin. 



The dog was very restless under the narcosis ; vomiting occurred twice. 

 Before the injection 37 grs of liver had been removed; 21.9 grs one and a 

 half hour after it. 



Amount of glycogen of the 1^* portion 9.035 %. 

 „ , 2"d , 6.683 0/,. 

 Loss 26o/o. 



TABLE I. 



Liquid injected 



Gain or Loss 

 of glycogen 



Time of 

 irrigation 



Particulars 



