254 



15. An alcoholic extract of the pancreas, the blood of which had been used 

 in the foregoing test was given to a large dog of 30 KG. The extract had been 

 prepared in the following manner: 20 grs of pancreas had been rubbed up and 

 macerated with 100 cc. of 65 ^o alcohol. During 'V^ hour this macerated substance 

 was evaporated down to Va of i^s volume at 65° C, made up to 180 cc. with 

 Locke's solution and filtered through wadding. 



The dog received this fluid during one hour through the jugular. 



The first portion removed from the fiver was 33 grs. 



The second (an hour later) 35V2 grs. 



Amount of glycogen of the 1^' portion 4.124 %. 

 „ „ „ „ „ 2d „ 4.356 0/0. 



The narcosis was remarkably restful, without any disturbance. 



In this experiment there was a gain of 57o- 



For an easy survey we summarize the results of this series of experiments in 

 the preceding table. 



CONCLUSIONS. 



Above all we observe that narcosis, laparotomy- and liver-extirpation, 

 in spite of continual irrigation bj injection of a fluid deemed favour- 

 able, result in a loss of glycogen. This is after all not surprising, 

 if we bear in mind that a number of glycolytic factors act upon 

 the liver. Among others: the narcosis per se, often associated with 

 considerable agitation; the animal's emotions; sometimes spasmodic 

 contractions caused by vomiting; loss of blood; fall of body-tempe- 

 rature. Nevertheless the fact deserves consideration in view of the 

 surgical process. The loss of glycogen averaged about 50 7o- Further- 

 more it follows from our experiments, that if defibrinated blood and 

 serum is given instead of Locke's solution, the decrease of glycogen 

 is considerably less. An injection of venous blood from a normal 

 dog lowers the amount of glycogen to about 29 7o i-^- about half 

 of the average. The average fall is still greater if irrigation is 

 accomplished with blood collected from an active pancreas. Then 

 the average decrease is only about 15 78» ie- Vs of the splitting 

 caused by irrigation with Locke's solution. 



Irrigation with normal blood, more particularly with pancreatic 

 blood, therefore, inhibits the loss of glycogen in the liver, nay in 

 one very successful experiment even a slight increment of glycogen 

 was noticeable. 



We have now to consider the problem as to how this result was 

 effected. Three hypotheses offer themselves for discussion. 



a. The blood favours glycogenosis. 



b. The blood impedes glycogenolysis. 



c. The blood neutralizes the inimical influence of the narcosis, 



