25!> 



As for the third theory it seems to me that the volume of the 

 injected blood and serum (about Vi„ of the blood of the body) is 

 too small to act vigorously. With regard to the other two, I do not 

 think it quite possible as yet to decide in favour of either of them. 

 Still, I incline towards the former, because occasionally an augmen- 

 tation of glycogen was to be noted, which on no account can be 

 originated by prevention of splitting alone. It is obvious that most 

 often the splitting pi-evailed over the formation of glycogen. 



Additional conclnsions from this series of experiments are the 

 following : 



In two cases an aqueous extract of the pancreas yielded negati\e 

 results; positive results, however, were obtained twice from an 

 alcoholic extract. Aqueous extracts and Locke's solution resulted in 

 an equivalent fall of the glycogen, alcoholic extracts in one instance 

 in 7» of the average, in another a rise of 5 "/„ was observed. It 

 seems then that the excitiny mjent of the pancreas is soluble in 

 alcohol, not in loater. It is probable therefore, that this agent is 

 present in the blood rather in physical adsorption than in chemical 

 solution. 



Again the thermostability was proved in both experiments. Lastly 

 we notify that a secretin deprived by alcohol of its vaso-dilating 

 substances acted favourably upon the amount of glycogen in the liver. 



Results compared loith those of De Meyer. 



Broadly speaking also this series of experiments lends support to 

 J. de Meyer's evidence. Our methods of investigation differ esseji- 

 tially, which renders the ultimate accordance in our results ail the 

 more satisfactory. My plan differs from de Meyer's 1. in experimenting 

 in vivo with all its drawbacks and advantages; 2. in the lack of 

 double circulations in the liver. 



The accordance in our results consists in : 



1. aiding the glycogenesis even through normal blood more than 

 through a physiological salt solution. 



2. a greater activity in this respect of blood that has passed 

 through the pancreas. 



It may also be remarked that the volume of the injected fluid is 

 considerably smaller than De Meyer uses in his experiments. It 

 should be borne in mind, however, that I worked in vivo, an 

 advantage of which is that glycogenetic factors are allowed to exert 

 an action beforehand, since a normal fluid containing sugar passes 

 through the liver. 



