262 



false apices. To all appearance the average ^'alue approximates the 

 ti'iith. It was moreover a mistake to make the second injection 

 after too short an interval from the first; this was done lest the 

 activity of -the substance should get lost; it would have been better 

 to inject both portions simultaneously. Yet also after these injections 

 a relatively slight fall of the D-secretion appears to take place, 

 which is still perceptible after a i'ew days. Furthermore a shoi't 

 increase of the D-percentage is to be noted especially after the 

 second injection by virtue of which the D/N coefticient rises con- 

 siderably. 



The results of the third and the fourth injections are still more 

 demonstrative. At the third the D-secrction during three days falls 

 short of what could be expected from the rise of the previous days. 

 Later on the excretion of sugar, likewise that of urine increases 

 rapidly. The changes in D- and 7V-concentriition are not conspicuous. 

 The fourth injection results in still more striking alterations. The 

 total Z)-secretion is lowered from 27 grs to 22 grs and 6 grs. The 

 quantity of urine diminishes from about 300 to 200 and 60 cc. 

 The Z)-percentage is suddenly exalted to rather more than 11 "/« ^"^^ 

 the Z)/JV -coefficient to 4,4, and 3,8 to fall again later on to 1,5. 

 Here then we have an accumulation of all the alterations announced 

 beforehand. Some particulars however must not be forgotten, viz. 

 after the 13^'* of October the dog declines nearly all food. However, 

 this fact does not in itself elucidate matters satisfactorily, since on 

 the 16'^' of October the sugar-secretion is normal again. 



Secondly the table shows that on the 12"^'^ of October acetone is 

 found in the urine for the first time, which no doubt will be attended 

 with other changes in the metabolism. Even this, however, does 

 not lessen the value of our results, seeing that acetonuria was on 

 the decrease and ultimately disappeared entirely, when the animal 

 was sinking rapidly and was sub flnem vitae on the 17^'' of October. 

 This proves that spontaneous lowering of the i)-secretion sub finem 

 vitae (a well-known phenomenon) must be carefully distinguished 

 from the D-secretion after injection, which contrariwise evolves a 

 separation of large quantities of acetone. 



We have to add in this connection, that we suspect the 4"^^ 

 injection to have been noxious to the animal's condition, which 

 was not surprising after all. At the section a considerable loss of 

 flesh was demonstrated ; there was no lipemia ; no trace of pancreatic 

 residue was found. 



We record one noteworthy experience. In examining the mouth 

 cavity of the animal bulbous tumours at the mucous membrane of 



