' 470 ) 



Fig. 3. Apparatus for rotting-experiment with water-current. A cylindrical glass 

 jar with flax V, B tube supplying water from the reservoir C to the bottom of .4, 

 D tube to draw off the water. T thermostat. 



pectose remains behind in tlie stalks. What is drawn ofT by D may 

 be called "rotting-water", but this water is widely different, at the 

 starting of the experiment, when it contains much dissolved matter 

 and few bacteria, from that, obtained in a later i»eriod, when many 

 bacteria and few dissolved substances occur in it. 



The jar A is kept at a temperature of 28 to 35'' C. by placing 

 it in the thermostat T. 



If after 2 or 3 days tiie tlax is removed from .1 it |)roves to 

 be more or less sufficiently i-otted, if the water sup|)ly has l)ecn 

 great enongli to refresh the rotting-water tV(Mii 5 to 10 times. Through 

 our apparatus of 300 cc, 1.5 to 3 1. of water had thus to |)ass. 

 Whilst in the Laboratory experiment the water is introduced at the 

 bottom of the jar and drawji otT at the toj) in order to prevent 

 stopping of the tubes l)y the gases formed by the fermentation, this 

 would be faulty with experiments on a lai'ger scale, — in this latter 

 case the heavier rottiim-water should be drained off at the bottom. 



