( «>09 ) 



applicjitioii are vcit satisfacloi-v. I li;i\(^ riii-llici- heen aI»lo lo develop 

 for the aclix ily of tlie cjr/jiues aii iiileiisityfortinila wliereiii, besides 

 the eoiiceiilralioiis of llie solutions, the constants found experimen- 

 tally oeciir and whieli I heliexe, g'ives a fair representation of the 

 (|uaiititative progress of llie enzym actions as studied l»y |)revions 

 observers and myself. 



4. These mathematical (l(Mluctions aJid llie details of the. other 

 results obtained by me will be published fully elsewhere. 1 nuiv 

 oidy remark fuiihei', that my results show thai, stai'liiig with a N/^ 

 saccharosesolution (171 grams per litre) we arrive at a final con- 

 dition where fully i gram of sacchai'ose still i-emains pi-esent. The 

 inversion therefore proceeds to an auiount greater than 99 "/„. This 

 seems also the case in the resolution <»f salicin. 



My experiments further show : 



l^*^ . that the two fei'meuts invertase and emulsiji retain their 

 quantitative activity for some weeks in properly stei'ilised solutions. 



2"'i. that the average factor for the change of the velocity of the 

 saccharose decomposition with the temperature equals 2 for 10° 

 between 0° and 25°. 



3'^^. that by a change of tem[»erature from 0^ to 25° the equili- 

 brium is displaced very little or not at all, which (piite agrees with 

 theory as the caloric eifect of the reaction is very small. 



4"^'^ . that at a definite temperature the change in concentration of 

 the enzymes has no influence on the equilibrium but only on the 

 velocity ; the latter, as in other catalytic actioiis, is directly proi)or- 

 tional to the enzyme concentration. 



5''' . that as already observed by Henri, the intensity of the 

 invertase is smaller in proportion as the quantity of imertsugar, 

 })resent with the same amount of saccharose, is greater. 



(3'''. that the intensity not only depends on the (piantity of invert- 

 sugar but also on the quantity of saccharose [)resent in the solution ; 

 it is smaller when this (piamity is greater. 



This last conclusion does iu)t agree with that of Hfari (Theses, 

 p. 72) where he states that the addition of saccharose at the 

 commencement of a reaction has lU) iidlnence on the \elocily of the 

 inversion, but accelerates it when the addition takes place in the 

 middle of a reaction. 



7^'^ that, as dnl}^ recpiired by theory, the reversal of the reactions 

 between glucose and fructose and between glucose and saligenin 

 })roceeds \ery slowly and requires se\cral weeks. 



The results of Hknri's experiments are in accordance with the 

 intensity formuki, which I have deduced. 



