54 NOTES ON FOWL ENTKRITIS 



EXPERIMENTS ON ANIMALS. 

 Tavo small black rabits, A and B, were inoculated (by means 

 of scarifying the ear) with a mere trace from a young 

 broth culture. Both apparently remained in perfect health 

 until the 11th day, when one suddenly appeared exhausted and 

 gradually sank into a drowsy condition for several hours and 

 then died. 



The other rabbit, after exhibiting the same symptoms, died 

 the following morning. 



Post-mortem in both cases was as follows : 

 Spleen. — The most noticeable feature was the enormous 

 enlargement of the spleen, which was of a dark purple 

 colour and firm in consistency. 

 Heart.— Normal in appearance, but cavities filled with 



coagulated blood. 

 Lungs. — Normal. 

 Liver. — Enlarged, friable, and slightly congested at the 



apices. 

 Intestines. ^Appeared normal. Faeces in the rectum showing 

 signs of diarrhoea. There was slight inflammation around 

 the seat of inoculation. 



MICROSCOPICAL EXAMINATION. 

 Spleen blood contained a fair number of bacilli. 

 Blood from the heart and lirer showed only very few 

 organisms. 



Six (6) tubes of Agar- Agar were inoculated from the heart, 

 liver, and spleen, all yielding a typical growth of the bacilli of 

 *' fowl enteritis." 



On October 26th I received two more dead fowls (Nos. 2 and 

 3) from Mr. Simpson. 



Post-mortem and microscopical examination gave positive 

 results. 



A small portion of the liver of one of the above fowls 

 (No. 3) was mashed up with a little broth and used for inoculat- 

 ing the following animals : — 

 1 young rooster, 

 1 rabbit, 



1 guinea pig, and 

 3 white rats. 



