4-1 TUBEKCULIN, ITS HISTOKV, PREPAKATION AND USE. 



Some tuberculin prepared in November, 1895, and kept 

 under favourable conditions, has not deteriorated in the slightest 

 degree, and is just as efficacious for diagnostic purposes as when 

 it was fir.st prepared. 



THE USE OF Trr.EECULIN AS A MEANS FOR 



DIAGONISING TUBERCULAR DISEASE 



IN CATTLE. 



Tuberculin as a test agent is always used in the diluted 

 form, in the proportion of one (1) part tuberculin to nineteen (19) 

 parts of a 5 in 1,000 Carbolic Acid solution. 



Of this diluted tuberculin the following doses are required 

 for different animals ; — 



8cc for bulls, high-class and aged animals. 

 6cc ,, medium-sized animals. 

 4cc ,, calves six to twelve months old. 

 2cc ,, calves under six months. 

 Example of method of diluting tuberculin : — l/lOcc pure 

 tuberculin to 7 6 lOcc carb sol. = 1 to 19. 



APPLIANCES REQUIRED. 



Si/rui'ie. — A strong well made hypodermic syringe holding 

 lOcc, with a strong needle attached, that used in connection with 

 preventive inoculation for Tick Fever is specially recommended. 



'L'hrniinincUr. — For taking the temperature a clinical ther- 

 mometer is necessary, as this form has a self-registering index, 

 but as the ordinary mi'dical thermometer is such an extremely 

 delicate instrument, and very liable to be broken, a special strong 

 thermometer, suitable for taking the temperature of cattle and 

 horses has been designeil and approved of by the Stock Insti- 

 tute. 



Thoroughly sterilize the syringe and needle {i.e. destroy all 

 germs that may be in or on them) by soaking them in 5 per cent, 

 solution of carbolic acid. 



THE APPLICATION OF THE TEST. 



The diagnoses of tuberculosis in cattle by means of this test 

 depends entirely upon the characteristic elevation of tempera- 

 ture produced in a tuberculo.sis animal within a limited period 

 after injection. 



