( 366 ) 



water of the temperature of the room and when the determination 

 is completed immediately afterwards. 



Van Hoogenhuyze and Yerploegh iiave investigated by themselves 

 whether increase of the secretion of creatinin in consequence of 

 muscular labour could be observed. For that purpose in every series 

 of experiments the urine was collected every day at appointed times 

 namely in the morning, in the first series of experiments at 9, in 

 the following at 8 o'clock, in the afternoon at 12 o'clock and at 

 J:7j o'clock, at night at 11 Vs o'clock. Every portion was measured 

 and divided into two equal halves. One of the halves was used for an 

 estimation of creatinin, the other halves were mixed, after which the 

 quantity of creatinin in the mixture was determined and moreover an 

 estimation of nitrogen was performed after the method of Kjeldahl. 



In this way the determination of creatinin was also controlled. In 

 all the series of experiments the conformity of the figure of the 

 total quantity of creatinin and the sum of the four portions was 

 very gratifying. The quantity of urine of one day was that collected 

 from 12 o'clock in the afternoon till the following morning 8 or 

 9 o'clock. 



During each series of experiments a fixed amount of food was 

 taken, every day the same. Only in the first series coffee and tea 

 were still taken, in the later series only water. 



I. From April the 8^'^ — 24*^1 1904, seventeen days at a stretch, food 

 was taken which consisted of bread, butter, cheese, milk, oatmeal, 

 sugar, meat, eggs, potatoes and rice, daily an equal portion of each. 



The food contained : 



for v. H. 118 gr. proteid 146 gr. fat, 326 gr. carbohydrat.; 40,8 Cal. p. Kg. 

 ,, Y. 115 ,, ,, 81 ,, ,, 327 ,, ,, 38.6 ,, ,, ,, 



On working days moreover both consumed 50 gr. sugar. 



The ll'h, the 16* and the 21* of April bicycle excursions were 

 undertaken at which they rode steadily on for 2V3 a 3 hours without 

 resting. The other days were spent in the laboratory while the 

 evenings were passed peacefully. 



The excretion of creatinin underwent no perceptible change in 

 consequence of the muscular labour. With both investigators it oscil- 

 lated not unimportantly during the whole experiment. It amounted 

 on an average to : 



V. H. 14 days of rest 2.116 gr. daily (max. 2,401, min. 1.821 gr.) 

 V. „ ,; „ „ 1.998 „ „' ( „ 2.158 „ 1.858 „) 



V. H. 3 workingdavs 2.147 „ „ („ 2.325 „ 1.925,,) 

 Y. „ „ 2.015 „ „ ( „ 2.058 „ 1.949 „ ) 



