( 375 ) 



circulation. He did find, ii is (rne, tlint after injection of creatin 

 nnder tlie skin, not only the whole injected qnantity was excreted 

 again with the urine, but also 20 mgr. creatinin witii it, but it 

 remained uncertain how much of it [)roceeded fi-om tlie metabolism 

 of the animal itself. 



To obtain an insight into this v. H. and V. have made anew 

 an experiment in which the same food was taken, with 47 gr. proteid 

 daily, as in the preceding experiment. 



VI. The experiment lasted from Aug. the 17''' till the 28 1'' J 905. 

 On the first day the urine was not examined. The oscillations in 

 the secretion of creatinin were very insignificant. In five days 

 from Aug. the IS"^'' till the 22"'^ there was secreted : 



V. H. average 2.023 gr. (max. 2.029, min. 2.017 gr.) daily 

 V. „ 2.028 „ ( „ 2.029, „ 1.930 „) „ 



On Aug. the 23''^^ each of them took in one portion 500 mgr. pure 

 creatinin dissolved in w\ater. On the same day there was excreted: 



V. H. 2.420 gr. and V. 2.508 gr. The next day: 

 V. 2.030 „ „ „ 2.073 „ „ 



On Aug. the 26''» each of them took again 500 mgr. creatinin but 

 divided into 10 portions, 50 mgr. every hour. Now also the creatinin 

 was found back the same day for the greater part in the urine. 

 The excretion amounted to : 



v. H. Aug. tlie 25^11 1.998 Aug. the 26''' 2.425 Aug. the 27^M.940 



Aug. the 28th 1.95J gi-. 



V. Aug. the 25t'> 2.045 Aug. the 26^'^ 2.467 Aug. the 27^^ 



2.035 Aug. tlie 28^'' 1.968 gr. 



At least in three of the four determinations a part of the creatinin 

 brought into tlie blood was not found back in tlie urine> 



From this experiment, wliicli has still to be completed with others, 

 in which creatin will be taken instead of creatinm, it appears how 

 distinctly every change of some importance in the excretion of crea^ 

 tinin can be shown with the aid of Folin's method. So it gives the 

 more reason to trust the results of the above mentioned series of 

 experiments, and tlie conclusion derived from them, that creatin is 

 a product of metabolism which is not formed at the contraction of 

 the muscle-fibre, but proceeds in muscles and other organs by the desin- 

 tegration of proteid to which is bound the life of the cells, without 

 regard to the developi)ig of energy to which they are able in 

 performing their peculiar functions. Oidy then when the organism 

 is deprived of food and must therefore seek the power of performing 



