284 
remained intact, less haemoglobin has been dissolved. The degree of 
haemolysis in these tubes is expressed by the proportion between the 
haemoglobin concentration in these tubes and the haemoglobin concen- 
tration of the tube with complete haemolysis. These proportions can 
be easily calculated by making use of the colorimetrical method 
first suggested by Arruenius’). Thus a series of values is obtained 
expressing which percentage of the complete haemolysis is caused 
by each salt-concentration. (Table A). The progress of this haemolysis 
may be expressed by a curve, the absciss of which is formed by 
the NaCl-concentrations and the ordinate by the degrees of haemo- 
lysis effected by each concentration. (Fig. 1). 
Haemolysis 
° 100% 
90% | 
80% | 
70% | 
60% + 
50% 
40% | 
dn 
50% Fr 
20% [ 
10% | 
— 
0.49% 051% 053% 0.55% 0.57% 059% 0.61% 0.63% We 
sol, 
Fig. 1. Graphical representation of Table A. 
TABLE A. Denoting the haemolysis caused by each 
NaCl concentration. 
0.63% NaCl solution caused 9% haemolysis 
0,61 » » > > 9 » > 
0.59 » » > > 9 > > 
0.57.9. va » > 42 » » 
0:55» > > 55 > » 
0.532 >» » » 131» » 
OF51 >a 2 > > 91 >» > 
0.49 » » > > 100 » » 
3. The capacity of resistance of old and new erythrocytes 
against diluted salt-solutions. 
The blood of an animal which has been made anaemic by bleeding, 
may be examined in the same way as that of a normal animal. 
1) ARRHENIUS and MADSEN, Zeilschr. für Physikal. Ghemie 1903. 
