292 
7. In the osmotic disturbance, caused by washing with 0.9°/, NaCl, 
the ions of Ca play a prominent part. 
This disturbance of the osmotic equilibrium of the blood-corpuscles, 
caused by washing with 0.9°/, NaCl, is not effected if we wash 
with 0.9°/, NaCl + 0.1 °/, CaCl,. What was pointed out before in 
the case of leucocytes, viz. the importance of ions of Ca’), is also 
found to apply to the erythrocytes. Though only traces of Ca are 
found in the erythrocytes, yet their capacity of resistance is con- 
siderably modified if osmose causes these few ions to disappear. 
Haem, 100% 
90% 
80% 
70% 
60% 
50% 
40% 
30% 
20% 
10% 
0.43% 0.45%  O47% 0.49% 0.51% 0.53% 0.55% 057% 0.59% NaCl 
Blood which has not been washed. ------- Blood washed with 0.9% NaCl 
ne Blood washed with 0.9% NaCl + 01% CaCl,. 
Fig. 5. Graphical representation of Table E. 
TABLE E. Blood which has been washed with a 0.9 pCt. NaCl-solution has a 
decreased capacity of resistance. 
Blood which has been washed with a 0.9 pCt. NaCl + 0.1 pCt. CaCl,- 
solution has „no decreased capacity of resistance. 
| 
Blood eee 
| | | 
ot been .weshed|with 8a pCi Nec (i ee Clee 
At 0.59 pCt. NaCl | En eld so a 
Gan mart Dar | = eee y oe | MIE 
Sen ee oe ele 35 10 pct. | 3 
B en EEA 60 DB 5 
BN NOP ae djs 18, 40 5 5 
Bee Ch eens 66, be 00, 60: Gals 
ERO AT Wee eS 14 100, 68 oF lhe 
RI tn big Gn = ee ES 
+043 08, ne 92 , = 0 , 15 
E04 Oh PS (0D = nT 
1) HAMBURGER and Hexma Biochem. Zeitschrift Bd. III and Bd. VIL. 
