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glycerin extract of the pancreas, liquids containing, besides lipase, a 
great quantity of other substances, chiefly proteins, and moreover 
some electrolytes. TERROINE has tried to remove the electrolytes from 
the pancreatic juice by dialysis, but this did not bring him nearer to 
his end, the dialysis causing the juice to lose its lipolytic activity. 
Rosenuyem has discovered’), that this was not due to deleterious 
action on the enzyme by the dialysis, nor to the diffusion of the 
lipase through the wall of the dialyser, but to the removal of a co- 
enzyme that readily diffuses, that withstands boiling and is soluble 
in alcohol. 
If the diffusate is evaporated and again added to the contents of 
the dialyser, its fat-splitting power is as great as before. The co-enzyme 
can be separated from the lipase not only by dialysis but also, as 
ROSENHEIM demonstrated, by diluting the glycerin extract of the 
pancreas with water, the result being a precipitate containing the 
enzyme, while the co-enzyme is left behind in solution. 
RosENHEIM’s suggestions induced me to use for my experiments 
lipase prepared in the following way: 
Fresh pig’s pancreas was minced up, then mixed with about twice 
its weight of glycerin and percolated after 24 hours. By filtration 
through a compressed pulp of filterpaper a solution can be obtained 
that is only slightly opalescent, whose lypolitie power however is far 
inferior to the original extract. Besides it yields after dilution with 
water a much smaller quantity of precipitate containing lipase. In 
preparing the enzyme I therefore used the extract only percolated 
through fine linen. This extract is highly opalescent, but little or no 
precipitate settles even after standing long. Part of this, mostly 30 ce, 
was mixed with ten times its quantity of distilled water. The liquid 
is very milky; however a satisfactory precipitate is not always 
obtained. 
To this effect a very faintly acid reaction, by addition of a few 
drops of diluted acetic acid, is required so as to colour sensitive 
blue litmuspaper faintly red. A stronger acid reaction would also 
cause a rather considerable amount of trypsin and trypsinogen to be 
precipitated. Next day the perfectly clear liquid is cautiously de- 
canted off from the residue and exchanged for 300 cc. of water; if 
necessary the water is acidulated with a few drops of acetic acid. 
After precipitation the decantation is repeated. The remaining fluid 
together with the precipitate is put on hardened paper in a BucHNeR 
filter and filtered off under pressure. The precipitate is repeatedly 
1) Proc. Physiol, Soc. Febr. 19, 1910, Journ. of Physiol, Vol. XL, 
