338 
washed in distilled water on the filter. It is then a greyish white 
mass which, after being thoroughly deprived of the superfluous water, 
can be easily removed from the filter and is now sufficiently free 
from electrolytes. After incineration the substance dried at 110°C. 
0.1521 erm. yielded 0.0004 gr. of ash 
and 0.2761 grim. yielded 0.0010 gr. of ash. 
The solution of this ash in boiling hydrochloric acid was yellow, 
which colour disappeared on dilution with water. This solution 
got vividly red with potassium sulphocyanate and did not give any 
calcium reaction. It was evident therefore, that the ash was chiefly 
composed of iron phosphate, which was not present before but only 
formed during incineration. 
The matter precipitated by dilution of the glycerin extract with 
water is soluble in highly dilute alkali. However, it also dissolves in 
elycerin without alkali. To effect this the precipitate, taken from the 
filter, was rubbed up in a mortar with pure glycerin. The solution 
gets clouded, nevertheless practically homogeneous. It preserves its acti- 
vity also after standing for a long time. Filtration makes it quite clear 
again, but deprives it of much of its activity. That is why I used 
the unfiltered solution. To dissolve the precipitate from 30 cc. of 
pancreatic extract, 20 cc. of glycerin was used, after which process 
the concentration of the enzyme — considering the inevitable loss of 
matter — was about equal to that of the original extract. The 
proteolytic and the amylolytic enzymes have been all but eliminated 
by the washing. The glycerin solution hardly attacks boiled starch 
and fibrin, not even after addition of some calcium chloride. It 
contains however a considerable amount of lipase. Still, the action 
of the enzyme is extremely weak without the addition of other 
substances. 
As RoseNueim detected, its activity is raised by mixing with the 
washwater (concentrated by evaporation), which has been separated 
from the precipitate, also when the evaporation occurs at a high 
temperature. Whereas in this respect Rosennui’s statements were 
fully confirmed by my experiments, I have been able to prove that, 
contrary to Rosgnnurm’s results, the power to aid the lipolysis is not 
lost through combustion. It is necessary, however, to dissolve the ash 
with a small quantity of boiling hydrochloric acid. When mixed 
with the neutralized solution, the glycerin solution of the enzyme 
(which further on I shall call only “‘lipase’’) evinces intense lipolysis. 
It is especially (though not exclusively) the calcium present in the 
ash that increases the activity of the enzyme. The bearing of very 
