341 
A CaCl... yk. PA ke ; Nao 
nan Ee ar te ak ee CO ae a 
III without addition Roce tr st) ae A ne pe 
Ee GEN On on ee AO er 
IV without addition STA Be Cte ea ee OLDER = 
ee DEET re EO ee eek ee AB = 
DOO 3 Je Dein vanden Wree A ee Dn 
lk 5, i EO IE NEE Coe eae je 
I was not astonished to find, that the lipolysis did not increase 
in the same degree as the amount of lime salt, nor that it did not 
increase regularly, since the quantity of fat contained in the gelat- 
inous deposit of calcium soap varies and in virtue of this a varying 
quantity of fat is abstracted from the influence of the enzyme. The 
precipitate also comprises free fatty acid, as it appeared necessary 
during titration to shake the fluid well. Thereby the tough calcium- 
soap was crumbling away and passed into coarse flakes collecting, 
after standing a short time, on the surface of the alcoholic fluid. 
When the calcium soap was broken up, alkali disappeared as was 
evident from the disappearance of the red colour. 
That CaCl, had indeed been decomposed by the fatty acid, may 
also be concluded from the greater amount of H-ions in the fluid, 
the determination of which I owe to Dr. RiNGer. 
A solution of 4 ee. of lipase with a little sodium carbonate in 
400 ce., was distributed in 4 bottles, and 1 ec. of neutral oil was 
added to each bottle. After digestion for 6 hours 50 ce. was pipetted 
off from each bottle to determine the H’-concentration. The remaining 
50 ec. containing about all the calcium soap was titrated. 
a without addition, 0.6 a NaHO, ez 8.1 & 10-8 
b with 10 mer. CaCl, 1.0 ,, » ey 6.6 X 10-7 
C 9) 25 o> bb] 1.4 23 be) 
Pee. TG, „CH 2.6 10-8 
The apparatus only allowed to work with three H-electrodes at 
a time, so that no H'-determination was made of c. 
Though the greater part of the titratable acid was left in the 
bottles, acidity, increasing with the amount of CaCl,, was distinctly 
noticeable in the fluid pipetted from 6 and d. Thus the fluid contained 
a strongly dissociated acid, which in this case was sure to be hydro- 
chloric acid. 
