342 
The lipolysis is also aided by lime salts that are very difficult to 
dissolve. 
4 ec. of lipase, after addition of 6 ee. of Na,CO, 0.2 °/,, diluted 
with water to 200 ee, is distributed in 4 bottles. To a only 1 ec. of 
neutral oil was added. To 6 moreover, 2 cc. of CaCl, 1°/,, to c 
2 ec. of CaCl, 1°/, as well as 3 cc. of an equivalent solution of 
K,C,O, and to d the centrifugalised washed precipitate obtained by 
mixing 2 ee. of CaCl, 1°/, with 3 cc. of the same solution of calcium 
oxalate. After digestion for six hours I found: 
‘ n 
a uses 0.2 ce. Zé NaHO 
h >) 2.0 2) 23 
C 9 0.9 9) 33 
d 3) 1.0 9) 33 
Calcium carbonate works likewise. The experiment also showed that 
CO, was set free. 
200 ee. solution of 4 ee. of lipase, with a little soda in 4 bottles 
a, b, ec, and d 50 ec. each. Beforehand I had put in e and d 
+ 200 mgr. of freshly precipitated CaCO, which was obtained by 
precipitating a water solution of 300 mer. of CaCl, with Na,CO, 
and repeatedly washing the precipitate with water in a centrifuge. 
The fluids, each with 1 ee. of oil, were digested for six hours. 
Immediately after this a and c were titrated. Through 5 and d a 
current of air free of CO, at 25° C. was passed for an hour and earried 
off through 50 ee.n/50 of barytic water. Subsequently also 6 and d 
were titrated. The barytic water through which the air in d was 
carried off had got very turbid, that of 6 hardly clouded. After 
precipitation of the barium carbonate formed, 40 cc. of the limpid 
fluid from each bottle was titrated with n/5 HCl. The result was: 
a uses 0.6 ce. - NaHO 
Be Send yields 0,14 ee. = CO, 
C 33 4.2 23 >) 
d 33 3.7 23 23 23 be) LAS 9) 9) 
A rather considerable quantity of carbonic acid had therefore been 
liberated from the calcium carbonate. The total acidity of d was 4.88, 
that of c being 4.2. Though, in the titration of the digested cloudy 
fluid, errors of 0.1, nay even of 0.2 cc. may possibly occur, this 
difference lies beyond the limit of the errors. The reason is obvious. 
While the air passed through the liquid it was heated to 25° C. to 
