343 
drive off the carbonic acid. Consequently the lipolysis could proceed 
again for the very reason, that by expelling the carbonic acid the 
equilibrium in the fluid was disturbed. Fatty acid could now be 
precipitated again through presence of the excess of calcium carbonate. 
As regards the action of calcium salts my results are not quite 
the same as those of Terrome, who found no or hardly any increase 
of the lipolysis by calcium chloride. The nature of TERROINE’s ex- 
periments however differed from mine. This experimenter made use 
of dog’s pancreatic juice of which 5 ee. was digested with 5 ce. of 
olive oil. This mixture, even without any addition, contained lime, 
and besides other electrolytes, a large quantity of colloid substances 
and comparatively little water, whereas for my researches the lipase, 
as much as possible freed from the other constituents of the pan- 
creatic extract, especially from the electrolytes, was dissolved in 
glycerin and strongly diluted with water. This method enabled me 
to study the action of the electrolytes all the better. 
Indeed, Terrome found the lipolysis increased after addition of 
magnesium- and barium chloride. This supports the belief that the 
action of the enzyme is promoted by precipitation of the liberated 
fatty acid. In this respect my results agreed with TrRROINE's as may 
appear from the following instances: 
Again 4 ce. of lipase was dissolved with a little sodium carbonate 
in water to a volume of 200 cc. and divided into four equal portions 
of 50 ec. To three of them equivalent quantities of CaCl,, BaCl, or 
MgCl, were added. The faint pink colour which disappeared, returned 
after the addition of some soda. 
After six hours’ digestion I used: 
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