044 
The magnesium soap which was formed, was not so tough and 
gelatinous as the calcium and the barium soap and could therefore 
not take up so much of the oil. Consequently less oil was protected 
against the action of the enzyme. I think the more powerful action 
of the magnesium chloride is owing to this fact. 
As known, sodium salts also aid the lipolysis. Here also, I think, 
the action is caused by the separation of fatty acid from the fluid, 
as insoluble soap. Sodium oleate is precipitated by solutions of different 
sodium salts of sufficient concentration, whereas in very weak 
salt solutions as well as in water they dissolve with opalescence. 
In order to arrive at an approximate estimation as to the degree of 
solubility, I made a solution of sodium oleate by dissolving pure 
oleic acid in alcohol and adding to it sufficient sodium hydrate to 
produce distinct alkaline reaction. Several mixtures were made of 
5 drops of this solution with 20 cc. of salt solutions varying in 
strength. This mixture was at once filtered. The filtrate was found 
to be less cloudy according as the precipitation had been more 
complete. 
NEO EEL AN eo ee Aree ee lear: 
B EE ee oe „ slightly opalescent 
NaBt Mon MN ete » Clear 
of Sete. hae GAARNE , Slightly opalescent 
A nn ELN „ “cloudy 
Nals TIMO Le EE „or «lear 
+ Dyin. a eee NEN eek: 5 eloudy 
NaF Dy arom IRN eames j; clear. 
Be AO EE » cloudy 
Cal. NOD Pek Jaret Ba eee » Clear 
en OMEN ee Pe MN „ Clear 
De ee eee = PR Bea » very cloudy 
Mol. OA os eg ee 5 «clear 
a OD oss os at EEE et » Clear 
i OMEN den eh Sera i. 3) CIC 
KCI AY) POE Ln nd » cloudy 
r 2) ay » | very cloudy 
By these researches the positive bearing of these salts (except 
that of Nal and NaF) on the lipolysis was ascertained. KCl, which 
does not precipitate soap by far so well as sodium salts, also exerted 
much less influence upon the lipolysis. The experiments were made 
in the usual way. Every time 50 cc. of a lipase and oil mixture, 
with or without addition of salt was digested for 6 hours and sub- 
sequently titrated. I used: 
