46 
keep the fluid well mixed during the pipetting even after shaking 
it thoroughly. Therefore mixtures of oleic acid and glycerin of the 
same composition as those that were to be digested, were prepared 
separately and immediately after the acidity was determined by 
titration. These samples were taken in duplicate in order to discover 
eventual errors in the measuring of the oleic acid. 
In every experiment I used: 10 ce. of glycerin, 2 cc. of oleic 
acid, 2 ee. of lipase and 3 ec. of toluol with or without addition 
of salt. The following are some of the results obtained : 
n : 
ce. — NaHO 
4 
Addition Immediately. After 24 hours. After 48 hours. 
Ie 0 be 17:2 13.9 
200 mer. CaCl, 23.1 23.4 
23.0 
Hi 0 Fe 18 19.5 
100 mer. CaCl, 23.3 23.0 
Addition. Immediately. After 24 hours 
23.5 Boe 
ill. 0 3.6 16.9 
10 mgr. CaCl, 19.3 
BO)” ¥ 20.4 
DUS 5 22.1 
: 23.6 5 
ING 0 Be 17.2 
10 mer. BrCl, 18.8 
DOS, 3 m 22.0 
TOOK. 5 23.7 
It is evident therefore that the synthesis was not increased. It was 
even strongly inhibited, just the reverse result as was obtained after 
addition of bile salts, prepared from oxbile after PLarrner’s method. 
1 5 
ec. — NaHO 
_ 4 
Addition. Immediately. After 10 hrs. After 24hrs. After 48 hrs. 
LL 0 ie 15.1 14.9 
100 mer. bilesalts 10.4 9.5 
u. 0 19.8 16.7 
100 mer. bilesalts. 14.2 11.2 
