DAY 
of Morrscn’s reagent a small aggregate of orange-yellow and red 
crystals appears in every cell; with little of the reagent I got large 
red and yellow aggregates of crystals in various places in the tissue 
or outside it. This last result need cause no surprise. The carotinoids 
are soluble in the solntion of the saponification-products formed and 
not in Motascn’s reagent. When a small quantity of the reagent is 
used no quick dilution of the soap-solution occurs and the carotinoids 
will have the chance of changing their place in the tissue. In general 
it is therefore preferable not to use too small a quantity of the reagent, 
unless for any reason large aggregates of crystals are desired. 
I have applied the potash method of Moniscu to the following 
objects. 
Flowers: Trollius caucasicus Stev., Nuphar luteum Sm., Chelido- 
nium majus L., Meconopsis cambrica Vig., Corydalis lutea DC., 
Erysimum Perofskianum Fisch. et Mey., Sinapis alba L., Isatis tine- 
toria L., Viola cornuta L. var. Daldowie Yellow, Cytisus sagittalis Koch 
(Genista sagittalis L.), Cytisus Laburnum L., Spartium junceum L., 
Thermopsis lanceolata R. Br., Cucurbita melanosperma A.Br., Ferula 
sp., Inula Helenium L., Doronicum Pardalianches L., Doronicum 
plantagineum L. excelsum, Calendula arvensis L., Taraxacum offici- 
nale Wigg., Hiéracium aurantiacum L., Hiéracium murorum L., 
Gazania splendens Hort., Asclepias curassavica L., Calceolaria rugosa 
Hook., Dendrobium thyrsiflorum Rehb. fil, Iris Pseudacorus  L., 
Narcissus Pseudonarcissus L., Clivia miniata Regel, Tulipa bortensis 
Gaerin., Fritillaria imperialis L., Lilium croceum Chaix, Hemerocallis 
Middendorffii Trautv. et Mey. 
Green leaves: Chelidonium majus L., Taraxacum officinale Wigg., 
Urtica dioica L., Triticum repens L., Selaginella Kraussiana A.Br. 
Yellow variegated leaves: Sambucus nigra L. fol. var., Grapto- 
phyllum pictum Griff., Croton ovalifolius Vahl. 
Fruits: Sorbus aucuparia L., Solanum Lycopersicum Trn. 
Root of Daucus Carota L. 
Algae: Cladophora sp., Spirogyra maxima (Hass.) Wittr., Haemato- 
coccus pluvialis Flot. 
It must be noted that in four of the above-named objects in their 
natural state carotinoids already occur in the form of crystals, namely 
in the root of Daucus Carota, the fruits of Sorbus aucuparia and 
of Solanum Lycopersicum, and in the flower of Clivia miniata. 
Acid method. 
By treating parts of green plants with dilute acids Frank *) observed 
1) A. Tscurrcu, Untersuchungen über das Chlorophyll, Landwirtsch. Jahrbücher, 
XIII. Bd., 1884, p. 490. Hans Moutscu, Le. p. 26. 
