521 
thing. For the non-green leaves and the parts which are not green; 
yet contain substances which belong to the carotinoids. How is it 
that these were not found by Tammes, whilst in other non-green 
parts of plants such as flowers, Tammrs obtained after some daysin 
all the eight cases investigated well-formed erystals which with 
reageuts showed the reactions proper to carotin. 1 am convinced by 
the use of Monisca’s reagent that carotinoids exist in the yellow 
parts of yellow variegated leaves. Sometimes I obtained separation 
of orange-yellow crystals, in other cases they were orange-yellow 
and red, but all gave the reaction proper to carotinoids. Kon *), 
with etiolated leaves, arrived at a different conclusion from that 
of Tammes. [I cannot refrain from remarking that Kour does not 
always correctly reproduce the results of Tammes, with whom he is 
in entire agreement. The following is quoted from Tammes ®): Ich 
habe auch gelbbunte, herbstlich gelbe und etiolirte Blatter in ver- 
diinnte Säurelösungen gebracht, aber stets mit negativen Resultaten. 
And from Koan*): Durch die neueren Untersuchungen der etiolirten 
Pflanzen mit Säuren, welche T. Tamames in grosser Zahl ausf ührte 
und welche ich, um in die unsicheren Anschauungen einige Klarheit 
zu bringen, planmässig fortgesetzt habe, ist es nun mit Sicherheit 
erwiesen, dass in allen etiolirten Pflanzenteilen, so weit sie gelb 
gefärbt, mit verdünnten Säuren Carotin-Krystalle zur Ausscheidung 
gebracht werden können. 
I treated objects, both with and without chlorophyll, such as green 
and yellow variegated leaves, yellow, orange-yellow, and orange 
flowers, and algae, with dilute acids at the ordinary temperature; 
namely, with 1°/,, 2°/, and 10°/, oxalic acid, 1°/, and 5°/, hydro= 
chlorie acid, 10°/, tartaric acid and 2°/, hydrofluorie acid solutions. 
The treatment often lasted one or two months. The objets which 
were subjected to this investigation, were the same as those investigated 
by the potash method of Mouiscu. 
In the case of green leaves I obtained with the dilute acids 
the above mentioned brown crystalline aggregates of a chlorophyll 
derivative which were formed in each cell containing chlorophyll, 
and here and there in the tissue red crystals, loose plates or 
aggregates. In the case of flowers, of which I investigated about 25, 
I generally obtained no crystals with dilute acids. Only in two 
cases was there a positive result, namely, in Asclepias curassavica;, 
where red crystals separated and in Calceolaria rugosa where orange- 
c.p. 48, - ; os 
GD, 220, ee hek on 2 
