690 
always become blue. With the solution of antimony trichloride all 
the crystals finally became dark-blue. Generally the colour is pure 
blue (Kr. and V. 426), but at the beginning of the reaction it is 
sometimes bluish-violet (476, 451). Occasionally in the use of con- 
centrated sulphuric acid, subsidiary phenomena appear, such as the 
flowing together of the crystals into blue drops, solution and separa- 
tion of blue droplets from the solution. 
When the crystals coloured blue by means of zine chloride or 
antimony trichloride are treated respectively with water or with 
dilute hydrochleric acid and water, the original orange-yellow or 
red colour reappears, although it may be somewhat less pure. 
Sulphurie acid and zine chloride solution in a more or less con- 
centrated state strongly attack the cell-walls, but this is much less 
so in the case of antimony trichloride solution. This to some extent 
may be reckoned an advantage of the latter reagent. 
There follows here a list of the organs and plants on which I 
have tested the two new reagents for carotinoids. 
Flowers: Trollius caucasicus Srrv., Chelidonium majus L., Isatis 
tinctoria L., Spartium junceum L., Thermopsis lanceolata R. Br., 
Cucurbita melanosperma A. Br., Ferulasp., Asclepias curassavica L., 
Calceolaria rugosa Hook., Dendrobium thyrsiflorum Reus. fil., Iris 
Pseudacorus I.., Narcissus Pseudonarcissus L., Lilium croceum CHarx. 
Green leaves: Chelidonium majus L., Urtica dioica L. 
Fruits: Sorbus aucuparia L., Solanum Lycopersicum Try. 
Root of Daucus Carota L. 
Algae: Cladophora sp., Haematococcus pluvialis Fror. 
Bromine. 
The behaviour of bromine water with respect to the carotinoid 
crystals was studied in about twenty cases. The crystals were for 
the most part separated out by using Moriscr’s reagent. Without 
exception a fugitive, greenish-blue colour was obtained. Generally 
the blue colour was clearly perceptible and only to a slight extent 
showed a greenish shade. In a few cases the coloration passed off 
quickly and the green predominated. This was so with Chelidonium 
majus and Spartium junceum. In the crystals of the fruit of 
Solanum Lycopersicum 1 saw the reddish-violet (Kr. and V. 581) 
colour successively change into bluish-violet (476), blue, greenish- 
blue (386) and green, and finally this last colour faded away. 
In the following cases, the crystals were investigated with bromine 
water. 
